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  Rapid SNARE-mediated fusion of liposomes and chromaffin granules with giant unilamellar vesicles.

Witkowska, A., & Jahn, R. (2017). Rapid SNARE-mediated fusion of liposomes and chromaffin granules with giant unilamellar vesicles. Biophysical Journal, 113(6), 1251-1259. doi:10.1016/j.bpj.2017.03.010.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-3070-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F11D-A
Genre: Journal Article

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 Creators:
Witkowska, A.1, Author              
Jahn, R.1, Author              
Affiliations:
1Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              

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 Abstract: Soluble N-ethylmaleimide-sensitive factor activating protein receptor (SNARE) proteins are the main catalysts for membrane fusion in the secretory pathway of eukaryotic cells. In vitro, SNAREs are sufficient to mediate effective fusion of both native and artificial membranes. Here we have established, to our knowledge, a new platform for monitoring SNARE-mediated docking and fusion between giant unilamellar vesicles (GUVs) and smaller liposomes or purified secretory granules with high temporal and spatial resolution. Analysis of fusion is restricted to the free-standing part of the GUV-membrane exhibiting low curvature and a lack of surface contact, thus avoiding adhesion-mediated interference with the fusion reaction as in fusion with supported bilayers or surface-immobilized small vesicles. Our results show that liposomes and chromaffin granules fuse with GUVs containing activated SNAREs with only few milliseconds delay between docking and fusion. We conclude that after initial contact in trans, SNAREs alone can complete fusion at a rate close to fast neuronal exocytosis.

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Language(s): eng - English
 Dates: 2017-04-082017-09-19
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.bpj.2017.03.010
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Title: Biophysical Journal
Source Genre: Journal
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Pages: - Volume / Issue: 113 (6) Sequence Number: - Start / End Page: 1251 - 1259 Identifier: -