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  A compendium of RNA-binding proteins that regulate microRNA biogenesis.

Treiber, T., Treiber, N., Plessmann, U., Harlander, S., Daiss, J. L., Eichner, N., et al. (2017). A compendium of RNA-binding proteins that regulate microRNA biogenesis. Molecular Cell, 66(2), 270-284. doi:10.1016/j.molcel.2017.03.014.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-33CF-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-33D9-8
Genre: Journal Article

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 Creators:
Treiber, T., Author
Treiber, N., Author
Plessmann, U.1, Author              
Harlander, S., Author
Daiss, J. L., Author
Eichner, N., Author
Lehmann, G., Author
Schall, K., Author
Urlaub, H.1, Author              
Meister, G., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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 Abstract: During microRNA (miRNA) biogenesis, two endonucleolytic reactions convert stem-loop-structured precursors into mature miRNAs. These processing steps can be posttranscriptionally regulated by RNA-binding proteins (RBPs). Here, we have used a proteomics-based pull-down approach to map and characterize the interactome of a multitude of pre-miRNAs. We identify similar to 180 RBPs that interact specifically with distinct pre-miRNAs. For functional validation, we combined RNAi and CRISPR/Cas-mediated knockout experiments to analyze RBP-dependent changes in miRNA levels. Indeed, a large number of the investigated candidates, including splicing factors and other mRNA processing proteins, have effects on miRNA processing. As an example, we show that TRIM71/LIN41 is a potent regulator of miR-29a processing and its inactivation directly affects miR-29a targets. We provide an extended database of RBPs that interact with pre-miRNAs in extracts of different cell types, highlighting a widespread layer of co- and posttranscriptional regulation of miRNA biogenesis.

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Language(s): eng - English
 Dates: 2017-04-202017-04-20
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.molcel.2017.03.014
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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 66 (2) Sequence Number: - Start / End Page: 270 - 284 Identifier: -