English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Ein fädiger DNS-Phage (fd) und ein sphärischer RNS-Phage (fr) wirtsspezifisch für männliche Stämme von E. coli

Hoffmann-Berling, H., Dürwald, H., Beulke, I., & Marvin, D. A. (1963). Ein fädiger DNS-Phage (fd) und ein sphärischer RNS-Phage (fr) wirtsspezifisch für männliche Stämme von E. coli. Zeitschrift für Naturforschung, B: A Journal of Chemical Sciences, 18(11), 893-898. doi:10.1515/znb-1963-1107.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-3D2F-5 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-3D3A-B
Genre: Journal Article
Alternative Title : Ein fädiger DNS-Phage und ein sphärischer RNS-Phage III Biologisches Verhalten

Files

show Files
hide Files
:
ZsNaturforschB_18_1963_893.pdf (Any fulltext), 6MB
 
File Permalink:
-
Name:
ZsNaturforschB_18_1963_893.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ZsNaturforschB_18_1963_876.pdf (Any fulltext), 8MB
 
File Permalink:
-
Name:
ZsNaturforschB_18_1963_876.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://www.degruyter.com/downloadpdf/j/znb.1963.18.issue-11/znb-1963-1107/znb-1963-1107.xml (Any fulltext)
Description:
-
OA-Status:
Locator:
https://doi.org/10.1515/znb-1963-1107 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Hoffmann-Berling, Hartmut1, Author           
Dürwald, Hildegard2, Author           
Beulke, Inge2, Author           
Marvin, Donald A., Author
Affiliations:
1Department of Molecular Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497702              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

Content

show
hide
Free keywords: -
 Abstract: fd and fr adsorb to male strains of E. coli and infect female cells, after the fd-DNA (or the fr-RNA 15) have been deproteinized by phenol and after the cells have been converted to the form of spheroplasts. fd is very heat resistant, highly antigenic and poorly adsorbing. The latency period of intracellular multiplication is 10 min (in Tryptone broth at 37 °C). The most unusual property seems to be that fd is the only phage on record which is liberated by the host cell without destruction of the host cell. The evidence for this is threefold: 1. in infected cultures phage is liberated at the rate of about 300 phage particles per bacterium per cell generation, and the growth rate of these cultures is indistinguishable from that of controls. 2. In such cultures no significant amounts of bacterial enzyme are liberated. 3. In single burst experiments it appears that more than 60% of the individual cells have liberated around 450 phage particles after about 20 min. and later produce bacterial growth as shown by turbidity. Non-lytic infection gives rise to an unstable carrier state. The phage is lost from the cells if superinfection is prevented by the addition of fd-antiserum. Lytic mutants of fd have been recorded. fr in its properties is similar to f2 and the other RNA phages and seems to be liberated by bacteriolysis, fr, due to the chemical nature of its nucleobases, is highly sensitive to hydroxylamine in slightly alkaline solution. Infection with fr in rare cases is non-lytic and leads to an unstable carrier state.

Details

show
hide
Language(s): deu - German
 Dates: 1963-04-262014-06-021963-11
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1515/znb-1963-1107
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Zeitschrift für Naturforschung, B: A Journal of Chemical Sciences
  Abbreviation : Z. Naturforsch., B: J. Chem. Sci.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Berlin : Walter de Gruyter GmbH
Pages: - Volume / Issue: 18 (11) Sequence Number: - Start / End Page: 893 - 898 Identifier: ISSN: 0932-0776
CoNE: https://pure.mpg.de/cone/journals/resource/954927655916