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  Roles of Sld2, Sld3, and Dpb11 in Replication Initiation

Reußwig, K.-U., Boos, D., & Pfander, B. (2016). Roles of Sld2, Sld3, and Dpb11 in Replication Initiation. In D. L. Kaplan (Ed.), The Initiation of DNA Replication in Eukaryotes (pp. 297-318). New York: Springer International Publishing.

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 Creators:
Reußwig, Karl-Uwe1, Author              
Boos, Dominik 2, Author
Pfander, Boris1, Author              
Affiliations:
1Pfander, Boris / DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565165              
2External Organizations, ou_persistent22              

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Free keywords: DNA replication initiation Origin firing Cell cycle regulation Temporal program of replication DNA damage checkpoint
 Abstract: Replication initiation in eukaryotes is subdivided into two phases—origin licensing and origin firing. These phases have opposite requirements for cyclin-dependent kinase (CDK) phosphorylation and are therefore restricted to different cell cycle phases: origin licensing occurs in the absence of CDK activity (G1 phase) and results in the formation of inactive precursors of the replicative DNA helicase at origins of replication. Origin firing occurs upon CDK activation in S phase and results in the activation of the replicative helicase. Central to the control of origin firing is a three-partite protein complex, which was first identified in budding yeast, but is apparently conserved among eukaryotes, and consists of Sld3, Dpb11, and Sld2 (SDS complex). Both Sld3 and Sld2 bind to Dpb11 in a CDK-phosphorylation-dependent manner and together form the minimal set of CDK substrates required for origin firing. The SDS complex facilitates helicase activation by promoting the association of accessory helicase subunits (Cdc45 and GINS complex) to form the CMG helicase. Importantly, the SDS complex-mediated CMG formation is a central element to various aspects of replication control, such as the temporal replication program and the regulation of origin firing by the checkpoint.

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 Dates: 2016
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-3-319-24696-3_15
 Degree: -

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Title: The Initiation of DNA Replication in Eukaryotes
Source Genre: Book
 Creator(s):
Kaplan, Daniel L., Editor
Affiliations:
-
Publ. Info: New York : Springer International Publishing
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 297 - 318 Identifier: ISBN: 978-3-319-24694-9