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  Measuring TGF-β Ligand Dynamics in Culture Medium

Feng, Z., Zi, Z., & Liu, X. (2016). Measuring TGF-β Ligand Dynamics in Culture Medium. In X.-H. Feng, P. Xu, & X. Lin (Eds.), TGF-β Signaling: methods and protocols. doi:10.1007/978-1-4939-2966-5_25.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-42D9-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-42DA-D
Genre: Book Chapter

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Feng.pdf (Publisher version), 468KB
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© Springer Science+Business Media New York 2016
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 Creators:
Feng, Zipei1, 2, Author
Zi, Zhike1, Author              
Liu, Xuedong2, Author
Affiliations:
1Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284              
2Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO, 80309, USA , ou_persistent22              

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Free keywords: TGF-β - Signaling - SMAD - Switch-like response
 Abstract: TGF-β plays an important role in a myriad of cell activities including differentiation, proliferation, and growth arrest. These effects are influenced by the concentration of TGF-β in the surrounding milieu, which is interpreted by mammalian cells and subsequently translated into meaningful signals that guide their proliferation, survival, or death. To predict cellular responses to TGF-ß signaling based on molecular mechanisms, it is important to consider how cells respond to different ligand doses and how variations in ligand exposure impact Smad signaling dynamics and subsequent gene expression. Here we describe methods to measure TGF-β concentration in the environment and approaches to perturb cellular TGF-β exposure to gain a quantitative understanding of signaling dynamics of this pathway.

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Language(s): eng - English
 Dates: 20162016
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1007/978-1-4939-2966-5_25
 Degree: -

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Title: TGF-β Signaling: methods and protocols
Source Genre: Book
 Creator(s):
Feng, Xin-Hua1, Editor
Xu, Pinglong2, Editor
Lin, Xia3, Editor
Affiliations:
1 Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, ou_persistent22            
2 Life Sciences Institute, Zhejiang University, ou_persistent22            
3 Department of Surgery, Baylor College of Medicine , ou_persistent22            
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISBN: 978-1-4939-2965-8
DOI: 10.1007/978-1-4939-2966-5

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Title: Methods in molecular biology
Source Genre: Series
 Creator(s):
Walker, John M.1, Editor
Affiliations:
1 School of Life and Medical Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK , ou_persistent22            
Publ. Info: New York : Springer
Pages: 394 Volume / Issue: 1344 Sequence Number: - Start / End Page: 379 - 389 Identifier: ISSN: 1064-3745