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  Conditional switch between frameshifting regimes upon translation of dnaX mRNA.

Caliskan, N., Wohlgemuth, I., Korniy, N., Pearson, M., Peske, F., & Rodnina, M. V. (2017). Conditional switch between frameshifting regimes upon translation of dnaX mRNA. Molecular Cell, 66(4), 558-567. doi:10.1016/j.molcel.2017.04.023.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-4C95-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-4C9E-1
Genre: Journal Article

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 Creators:
Caliskan, N.1, Author              
Wohlgemuth, I.1, Author              
Korniy, N.1, Author              
Pearson, M.1, Author              
Peske, F.1, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578598              

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Free keywords: dnaX; kinetics; programmed ribosome frameshifting; protein synthesis; recoding; tRNA abundance; translation pausing; translation regulation
 Abstract: Ribosome frameshifting during translation of bacterial dnaX can proceed via different routes, generating a variety of distinct polypeptides. Using kinetic experiments, we show that -1 frameshifting predominantly occurs during translocation of two tRNAs bound to the slippery sequence codons. This pathway depends on a stem-loop mRNA structure downstream of the slippery sequence and operates when aminoacyl-tRNAs are abundant. However, when aminoacyl-tRNAs are in short supply, the ribosome switches to an alternative frameshifting pathway that is independent of a stem-loop. Ribosome stalling at a vacant 0-frame A-site codon results in slippage of the P-site peptidyl-tRNA, allowing for -1-frame decoding. When the -1-frame aminoacyl-tRNA is lacking, the ribosomes switch into -2 frame. Quantitative mass spectrometry shows that the -2-frame product is synthesized in vivo. We suggest that switching between frameshifting routes may enrich gene expression at conditions of aminoacyl-tRNA limitation.

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Language(s): eng - English
 Dates: 2017-05-18
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.molcel.2017.04.023
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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 66 (4) Sequence Number: - Start / End Page: 558 - 567 Identifier: -