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  Determining the bacterial cell biology of Planctomycetes

Boedeker, C., Schüler, M., Reintjes, G., Jeske, O., van Teeseling, M. C. F., Jogler, M., et al. (2017). Determining the bacterial cell biology of Planctomycetes. Nature Communications, 8: 14853. doi:10.1038/ncomms14853.

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 Creators:
Boedeker, Christian1, Author
Schüler, Margarete2, Author              
Reintjes, Greta1, Author
Jeske, Olga1, Author
van Teeseling, Muriel C. F.1, Author
Jogler, Mareike1, Author
Rast, Patrick1, Author
Borchert, Daniela1, Author
Devos, Damien P.1, Author
Kucklick, Martin1, Author
Schaffer, Miroslava2, Author              
Kolter, Roberto1, Author
van Niftrik, Laura1, Author
Engelmann, Susanne1, Author
Amann, Rudolf1, Author
Rohde, Manfred1, Author
Engelhardt, Harald2, Author              
Jogler, Christian1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: CANDIDATUS KUENENIA STUTTGARTIENSIS; GRAM-NEGATIVE BACTERIA; GEMMATA-OBSCURIGLOBUS; SUBCELLULAR-LOCALIZATION; ESCHERICHIA-COLI; OUTER-MEMBRANE; CRYOELECTRON TOMOGRAPHY; PSEUDOMONAS-AERUGINOSA; ENDOPLASMIC-RETICULUM; BIOACTIVE MOLECULESScience & Technology - Other Topics;
 Abstract: Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms.

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Language(s): eng - English
 Dates: 2017-04-10
 Publication Status: Published online
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000398924900001
DOI: 10.1038/ncomms14853
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 14853 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723