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  Large-Scale Mitotic Cell Synchronization

Dulla, K., & Margalef, A. S. (2017). Large-Scale Mitotic Cell Synchronization. In CELL CYCLE SYNCHRONIZATION: METHODS AND PROTOCOLS, 2ND EDITION (pp. 65-74). 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA: HUMANA PRESS INC.

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 Creators:
Dulla, Kalyan1, Author              
Margalef, Anna Santamaria2, Author
Affiliations:
1Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              
2external, ou_persistent22              

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Free keywords: QUANTITATIVE PHOSPHOPROTEOMICS; PHOSPHORYLATION; CYCLE; CDK1Biochemistry & Molecular Biology; Cell Biology; Mitosis; Large-scale synchronization; HeLa S3; HeLa S; Spinner cultures; Triple flasks; Prometaphase; Metaphase; Anaphase/telophase;
 Abstract: Understanding cell growth and cell division involves the study of regulatory events that occur in a cell cycle phase-dependent manner. Studies analyzing cell cycle regulatory mechanisms and cell cycle progression invariably require synchronization of cell populations at specific cell cycle stages. Several methods have been established to synchronize cells, including serum deprivation, contact inhibition, centrifugal elutriation, and drug-dependent synchronization. Despite potential adverse cellular consequences of synchronizing cells by pharmacological agents, drug-dependent methods can be advantageous when studying later cell cycle events to ensure specific enrichment at selected mitotic stages. This chapter describes protocols used in our laboratory for isolating mitotic mammalian cells in a large-scale manner. In particular, we discuss the technical aspects of adherent or suspension cell isolation, the methods necessary to enrich cells at different mitotic stages and the optimized culture conditions.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: CELL CYCLE SYNCHRONIZATION: METHODS AND PROTOCOLS, 2ND EDITION
  Alternative Title : Methods Mol. Biol.
  Alternative Title : METHODS MOL BIOL
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA : HUMANA PRESS INC
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 65 - 74 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-6603-5; 978-1-4939-6602-8

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Title: Methods in Molecular Biology
  Alternative Title : METHODS MOL BIOL
  Alternative Title : Methods Mol. Biol.
Source Genre: Series
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Publ. Info: -
Pages: - Volume / Issue: 1524 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745