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  MPI tray: a versatile crystallization plate for membrane proteins

Rathmann, B., Quirnheim Pais, D., & Thielmann, Y. (2017). MPI tray: a versatile crystallization plate for membrane proteins. Journal of Applied Crystallography, 50(1), 327-330. doi:10.1107/S1600576716019452.

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 Creators:
Rathmann, Barbara1, Author           
Quirnheim Pais, David1, Author           
Thielmann, Yvonne1, Author           
Affiliations:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: crystallization plates; surface treatment; UV imaging; membrane proteins
 Abstract: High-throughput crystallization of biological macromolecules is usually performed on multi-well plates, the design of which needs to address different and sometimes conflicting requirements. In this regard, handling of membrane proteins presents a particular challenge owing to the common use of detergents with associated effects on surface tension. Reported here is the design of a new crystallization plate, termed the MPI tray, which is optimized for UV and visible imaging with membrane protein samples. Following basic considerations regarding geometry and material, the surface properties of the plate were subjected to extensive analysis and modification in order to improve the performance in a robotic environment. An electrostatic surface potential was identified as the major problem affecting the automated setup of experiments, and it was found that treatment of the crystallization plate with ethanol is effective in removing this potential.

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Language(s): eng - English
 Dates: 2016-09-232016-12-052017-02
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S1600576716019452
 Degree: -

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Title: Journal of Applied Crystallography
  Abbreviation : J. Appl. Cryst.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford, England : Blackwell Publishing on behalf of the International Union of Crystallography
Pages: - Volume / Issue: 50 (1) Sequence Number: - Start / End Page: 327 - 330 Identifier: ISSN: 0021-8898
CoNE: https://pure.mpg.de/cone/journals/resource/954925410812