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  Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp

Jia, X.-H., Zhang, C.-L., Shi, D.-J., Zhuang, M.-M., Wang, X., Jia, R., et al. (2016). Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp. Journal of applied phycology, 28(2), 1001-1009. doi:10.1007/s10811-015-0607-4.

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 Creators:
Jia, Xiao-Hui1, Author
Zhang, Chun-Li2, Author              
Shi, Ding-Ji1, 3, Author
Zhuang, Min-Min1, Author
Wang, Xiang1, Author
Jia, Rui1, Author
Zhang, Zheng-Yang1, Author
Huang, Jie4, Author
Sun, Yi-Hua1, Author
Qian, Wne-Yi1, Author
Peng, Guo-Hong2, Author              
He, Pei-Min1, Author
Affiliations:
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education of Shanghai, Shanghai Ocean University, Shanghai 201306, China, ou_persistent22              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              
3Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China, ou_persistent22              
4Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China, ou_persistent22              

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Free keywords: Anabaena sp. PCC 7120; Cyanobacteria; White spot syndrome virus (WSSV); vp28 gene expression; Oral vaccine; Photosynthesis
 Abstract: White spot syndrome virus (WSSV) is one of the most prevalent and lethal viruses for shrimp, and oral administration of VP28 is a promising approach to protect shrimp against WSSV. Although seven heterologous expression systems have been successful since 2004, no one has been commercialized in shrimp industry. This paper presented the idea of “both drug and feed from the same feedstock” to develop oral vaccine against WSSV. Filamentous, heterocystous cyanobacterium, Anabaena sp. PCC 7120, was used to express major envelope protein, VP28, for feeding post larvae of shrimp. The expression of vp28 gene in transgenic mutant was analyzed by Western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). For vaccination, Litopenaeus vannamei was orally administrated by feeding the wild type and mutants of Anabaena cells for 10 days, and was challenged with WSSV during 10 days post vaccination. The post larvae were fed with the mutant harboring vp28 (10 μg shrimp1). The post larvae were divided five groups and their survivals were as follows: (1) negative control (neither vaccination nor challenge), 100%; (2) positive control (challenge and no vaccination), 0%; (3) feeding the wild type, 15.5%; (4) feeding blank, 20.6%; and (5) feeding the mutant harboring vp28, 68.0%. Moreover, the post larvae fed the mutant harboring vp28 grew better than no feed. Also, the photosynthetic features indicated the transgenic mutant was easier to cultivate. These data show that transgenic mutant harboring vp28 was favorable not only to use both for drug and feed but also to cultivate in scale.

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Language(s): eng - English
 Dates: 2014-11-302015-04-272015-05-152016-04
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s10811-015-0607-4
 Degree: -

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Title: Journal of applied phycology
  Abbreviation : J Appl Phycol
Source Genre: Journal
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Publ. Info: Dordrecht [u.a.] : Springer Science + Business Media B.V
Pages: - Volume / Issue: 28 (2) Sequence Number: - Start / End Page: 1001 - 1009 Identifier: Other: 1573-5176
CoNE: https://pure.mpg.de/cone/journals/resource/1573-5176