English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Conformational dynamics and allostery in E2:E3 interactions drive ubiquitination: gp78 and Ube2g2.

Chakrabarti, K. S., Li, J., Das, R., & Byrd, R. A. (2017). Conformational dynamics and allostery in E2:E3 interactions drive ubiquitination: gp78 and Ube2g2. Structure, 25(5), 794-805. doi:10.1016/j.str.2017.03.016.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-598A-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-5991-3
Genre: Journal Article

Files

show Files
hide Files
:
2450903.pdf (Publisher version), 5MB
 
File Permalink:
-
Name:
2450903.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450903_Suppl_1.pdf (Supplementary material), 681KB
Name:
2450903_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450903_Suppl_2.pdf (Supplementary material), 220KB
Name:
2450903_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450903_Suppl_3.pdf (Supplementary material), 171KB
Name:
2450903_Suppl_3.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450903_Suppl_4.pdf (Supplementary material), 5MB
Name:
2450903_Suppl_4.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Chakrabarti, K. S.1, Author              
Li, J., Author
Das, R., Author
Byrd, R. A., Author
Affiliations:
1Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: -
 Abstract: Conformational dynamics plays a fundamental role in molecular recognition and activity in enzymes. The ubiquitin-conjugating enzyme (E2) Ube2g2 functions with the ubiquitin ligase (E3) gp78 to assemble poly-ubiquitin chains on target substrates. Two domains in gp78, RING and G2BR, bind to two distant regions of Ube2g2, and activate it for ubiquitin (Ub) transfer. G2BR increases the affinity between the RING and Ube2g2 by 50-fold, while the RING catalyzes the transfer of Ub from the Ube2g2 similar to Ub conjugate. How G2BR and RING activate Ube2g2 is unclear. In this work, conformational dynamics in Ube2g2 revealed a clear correlation of binding G2BR and RING with the sequential progression toward Ub transfer. The interrelationship of the existence and exchange between ground and excited states leads to a dynamic energy landscape model, in which redistribution of populations contributes to allostery and activation. These findings provide insight into gp78's modulation of conformational exchange in Ube2g2 to stimulate ubiquitination.

Details

show
hide
Language(s): eng - English
 Dates: 2017-05-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.str.2017.03.016
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Structure
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 25 (5) Sequence Number: - Start / End Page: 794 - 805 Identifier: -