English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Proteomic profiling of human intraschisis cavity fluid.

Sudha, D., Kohansalnodehi, M., Kovuri, P., Manda, S. S., Neriyanuri, S., Gopal, L., et al. (2017). Proteomic profiling of human intraschisis cavity fluid. Clinical Proteomics, 14: 13. doi:10.1186/s12014-017-9148-y.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-5A61-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-51B1-B
Genre: Journal Article

Files

show Files
hide Files
:
2450999.pdf (Publisher version), 2MB
Name:
2450999.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2450999_Suppl_1.xlsx (Supplementary material), 164KB
Name:
2450999_Suppl_1.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450999_Suppl_2.tif (Supplementary material), 901KB
Name:
2450999_Suppl_2.tif
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450999_Suppl_3.tif (Supplementary material), 2MB
Name:
2450999_Suppl_3.tif
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450999_Suppl_4.tif (Supplementary material), 844KB
Name:
2450999_Suppl_4.tif
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2450999_Suppl_5.tif (Supplementary material), 148KB
Name:
2450999_Suppl_5.tif
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Sudha, D., Author
Kohansalnodehi, M.1, Author              
Kovuri, P., Author
Manda, S. S., Author
Neriyanuri, S., Author
Gopal, L., Author
Bhende, P., Author
Chidambaram, S., Author
Arunachalam, J. P., Author
Affiliations:
1Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              

Content

show
hide
Free keywords: Intraschisis fluid; X-linked retinoschisis; Proteome; Pathway analysis; Immune response; Inflammation
 Abstract: Background: X-linked retinoschisis (XLRS) is a vitreoretinal degenerative disorder causing vision deterioration, due to structural defects in retina. The hallmark of this disease includes radial streaks arising from the fovea and splitting of inner retinal layers (schisis). Although these retinal changes are attributed to mutations in the retinoschisin gene, schisis is also observed in patients who do not carry mutations. In addition, the origin of intraschisis fluid, the triggering point of schisis formation and its progression are largely unknown still. So far, there is no report on the complete proteomic analysis of this fluid. Schisis fluid proteome could reflect biochemical changes in the disease condition, helping in better understanding and management of retinoschisis. Therefore it was of interest to investigate the intraschisis fluid proteome using high-resolution mass spectrometry. Methods: Two male XLRS patients (aged 4 and 40 years) underwent clinical and genetic evaluation followed by surgical extraction of intraschisis fluids. The two fluid samples were resolved on a SDS-PAGE and the processed peptides were analyzed by Q-Exactive plus hybrid quadrupole-Orbitrap mass spectrometry. Functional annotation of the identified proteins was performed using Ingenuity pathway analysis software. Results: Mass spectrometry analysis detected 770 nonredundant proteins in the intraschisis fluid. Retinol dehydrogenase 14 was found to be abundant in the schisis fluid. Gene ontology based analysis indicated that 19% of the intraschisis fluid proteins were localized to the extracellular matrix and 15% of the proteins were involved in signal transduction. Functional annotation identified three primary canonical pathways to be associated with the schisis fluid proteome viz., LXR/RXR activation, complement system and acute phase response signalling, which are involved in immune and inflammatory responses. Collectively, our results show that intraschisis fluid comprises specific inflammatory proteins which highly reflect the disease environment. Conclusion: Based on our study, it is suggested that inflammation might play a key role in the pathogenesis of XLRS. To our knowledge, this is the first report describing the complete proteome of intraschisis fluid, which could serve as a template for future research and facilitate the development of therapeutic modalities for XLRS.

Details

show
hide
Language(s): eng - English
 Dates: 2017-04-242017-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1186/s12014-017-9148-y
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Clinical Proteomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 12 Volume / Issue: 14 Sequence Number: 13 Start / End Page: - Identifier: -