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  Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex.

Xu, Y., Bernecky, C., Lee, C. T., Maier, K. C., Schwalb, B., Tegunov, D., et al. (2017). Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex. Nature Communications, 8: 15741. doi:10.1038/ncomms15741.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-5BA8-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-5BAD-8
Genre: Journal Article

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 Creators:
Xu, Y.1, Author              
Bernecky, C.1, Author              
Lee, C. T.2, Author              
Maier, K. C.1, Author              
Schwalb, B.1, Author              
Tegunov, D.1, Author              
Plitzko, J. M., Author
Urlaub, H.2, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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 Abstract: The conserved polymerase-associated factor 1 complex (Paf1C) plays multiple roles in chromatin transcription and genomic regulation. Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II (Pol II) transcription elongation complex (EC). Here we report the reconstitution of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound to a Pol II EC containing the elongation factor TFIIS. Cryo-electron microscopy and crosslinking data reveal that Paf1C is highly mobile and extends over the outer Pol II surface from the Rpb2 to the Rpb3 subunit. The Paf1-Leo1 heterodimer and Cdc73 form opposite ends of Paf1C, whereas Ctr9 bridges between them. Consistent with the structural observations, the initiation factor TFIIF impairs Paf1C binding to Pol II, whereas the elongation factor TFIIS enhances it. We further show that Paf1C is globally required for normal mRNA transcription in yeast. These results provide a three-dimensional framework for further analysis of Paf1C function in transcription through chromatin.

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Language(s): eng - English
 Dates: 2017-06-06
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/ncomms15741
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Title: Nature Communications
Source Genre: Journal
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Pages: 13 Volume / Issue: 8 Sequence Number: 15741 Start / End Page: - Identifier: -