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  An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly.

Jakhanwal, S., Lee, C. T., Urlaub, H., & Jahn, R. (2017). An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly. EMBO Journal, 36(12), 1788-1802. doi:10.15252/embj.201696270.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-5E6D-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-39D5-F
Genre: Journal Article

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 Creators:
Jakhanwal, S.1, Author              
Lee, C. T.2, Author              
Urlaub, H.2, Author              
Jahn, R.1, Author              
Affiliations:
1Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              
2Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: SNARE ; Munc18; NSF; SM‐protein; intermediates
 Abstract: Assembly of the SNARE proteins syntaxin1, SNAP25, and synaptobrevin into a SNARE complex is essential for exocytosis in neurons. For efficient assembly, SNAREs interact with additional proteins but neither the nature of the intermediates nor the sequence of protein assembly is known. Here, we have characterized a ternary complex between syntaxin1, SNAP25, and the SM protein Munc18-1 as a possible acceptor complex for the R-SNARE synaptobrevin. The ternary complex binds synaptobrevin with fast kinetics, resulting in the rapid formation of a fully zippered SNARE complex to which Munc18-1 remains tethered by the N-terminal domain of syntaxin1. Intriguingly, only one of the synaptobrevin truncation mutants (Syb1-65) was able to bind to the syntaxin1:SNAP25:Munc18-1 complex, suggesting either a cooperative zippering mechanism that proceeds bidirectionally or the progressive R-SNARE binding via an SM template. Moreover, the complex is resistant to disassembly by NSF Based on these findings, we consider the ternary complex as a strong candidate for a physiological intermediate in SNARE assembly.

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Language(s): eng - English
 Dates: 2017-05-082017-06-14
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.15252/embj.201696270
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Title: EMBO Journal
Source Genre: Journal
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Pages: - Volume / Issue: 36 (12) Sequence Number: - Start / End Page: 1788 - 1802 Identifier: -