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  Calcium ion-dependent myofibrillar adenosine triphosphatase activity correlated with the contractile response

Hasselbach, W., & Murphy, R. A. (1968). Calcium ion-dependent myofibrillar adenosine triphosphatase activity correlated with the contractile response. The Journal of Biological Chemistry, 243, 5656-5662.

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Alternativer Titel : Calcium Ion-dependent Myofibrillar Adenosine Triphosphatase Activity Correlated with the Contractile Response TEMPERATURE-INDUCED LOSS OF CALCIUM ION SENSITIVITY

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 Urheber:
Hasselbach, Wilhelm1, Autor           
Murphy, R. A., Autor
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Zusammenfassung: We investigated the effect of temperature on (a) calcium ion binding by ethylene glycol bis(β-aminoethyl ether)-N,N'-tetraacetic acid (EGTA), (b) the calcium ion dependence of rabbit skeletal muscle myosin B and myofibrillar adenosine triphosphatase activity, and (c) myofibrillar syneresis. Temperature changes between 0° and 40° had no significant effects on calcium ion concentrations established by an EGTA-CaEGTA buffer system. Both initial and basic rates of myosin B ATPase activity showed a constant calcium ion requirement from 20° to 40° with [Ca++] for half-maximal activity of 7.1 ± 1.0 x 10-7 m. During a brief initial period the rate of myofibrillar ATPase activity showed the same calcium ion dependence. The initial high rate of calcium ion-dependent myofibrillar ATPase activity was associated with a rapid increase in absorbance, which lasted for about 1 min at 20° and only 20 set at 37°. After the initial rapid increase in absorbance was complete, the rate of calcium ion-dependent myofibrillar ATPase activity declined (although considerable calcium ion-insensitive ATPase activity remained). The extent to which calcium ion-dependent myofibrillar ATPase activity was lost increased with the extent of syneresis, both factors being promoted by an increase in temperature. At all temperatures there was a correlation between the initial calcium ion-dependent rate of myofibrillar ATPase activity and the over-all rate of the initial rapid increase in absorbance. The decrease in calcium ion-dependent ATPase activity in myofibrils, but not in myosin B, following the rapid phase of syneresis appears to be a consequence of the contractile event.

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Sprache(n): eng - English
 Datum: 1968-06-171968-11-10
 Publikationsstatus: Erschienen
 Seiten: 8
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Titel: The Journal of Biological Chemistry
  Andere : JBC
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Seiten: - Band / Heft: 243 Artikelnummer: - Start- / Endseite: 5656 - 5662 Identifikator: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1