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  Initiation and termination events in double stranded ϕx-DNA replication

Knippers, R., & Müller-Wecker, H. (1970). Initiation and termination events in double stranded ϕx-DNA replication. The FEBS Journal, 15(1), 146-154. doi:10.1111/j.1432-1033.1970.tb00989.x.

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FEBSJ_15_1970_146.pdf (Any fulltext), 784KB
 
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 Creators:
Knippers, Rolf1, Author           
Müller-Wecker, Hildegard1, Author           
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1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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 Abstract: The experiments reported in this paper lead to the following conclusions:

1An endonucleolytic activity is associated with the “replication site” in Escherichia coli cells; this endonucleolytic activity attacks the complementary strand of the ϕ x replicative form DNA only.
2The viral strand of the ϕ x-replicative form DNA remains circularly closed during the replication cycle;
3In the immediate replication product both composing strands are linear; in circularization no strand is prefered;
4A replication product with both strands closed is transiently found in a structural state which can be, under our experimental conditions, only partially denatured by alkali. Subsequently it is transformed to a typical supercoiled RFI structure.

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Language(s): eng - English
 Dates: 1970-03-271970-05-112005-03-031970-07
 Publication Status: Published in print
 Pages: 9
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 Table of Contents: -
 Rev. Type: Peer
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Title: The FEBS Journal
  Other : The Federation if European Biochemical Societies Journal
Source Genre: Journal
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Publ. Info: Wiley-Blackwell
Pages: - Volume / Issue: 15 (1) Sequence Number: - Start / End Page: 146 - 154 Identifier: ISSN: 1742-464X
CoNE: https://pure.mpg.de/cone/journals/resource/954925398485