English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Ribosome rearrangements at the onset of translational bypassing.

Agirrezabala, X., Samatova, E. N., Klimova, M., Zamora, M., Gil-Carton, D., Rodnina, M. V., et al. (2017). Ribosome rearrangements at the onset of translational bypassing. Science Advances, 3(6): e1700147. doi:10.1126/sciadv.1700147.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-7F87-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-7F8A-9
Genre: Journal Article

Files

show Files
hide Files
:
2457090.pdf (Publisher version), 2MB
Name:
2457090.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Agirrezabala, X., Author
Samatova, E. N.1, Author              
Klimova, M.1, Author              
Zamora, M., Author
Gil-Carton, D., Author
Rodnina, M. V.1, Author              
Valle, M., Author
Affiliations:
1Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578598              

Content

show
hide
Free keywords: bypassing; recoding; ribosome; structure; translation
 Abstract: Bypassing is a recoding event that leads to the translation of two distal open reading frames into a single polypeptide chain. We present the structure of a translating ribosome stalled at the bypassing take-off site of gene 60 of bacteriophage T4. The nascent peptide in the exit tunnel anchors the P-site peptidyl-tRNAGly to the ribosome and locks an inactive conformation of the peptidyl transferase center (PTC). The mRNA forms a short dynamic hairpin in the decoding site. The ribosomal subunits adopt a rolling conformation in which the rotation of the small subunit around its long axis causes the opening of the A-site region. Together, PTC conformation and mRNA structure safeguard against premature termination and read-through of the stop codon and reconfigure the ribosome to a state poised for take-off and sliding along the noncoding mRNA gap.

Details

show
hide
Language(s): eng - English
 Dates: 2017-06-07
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/sciadv.1700147
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science Advances
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 8 Volume / Issue: 3 (6) Sequence Number: e1700147 Start / End Page: - Identifier: -