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  Ribosome rearrangements at the onset of translational bypassing.

Agirrezabala, X., Samatova, E. N., Klimova, M., Zamora, M., Gil-Carton, D., Rodnina, M. V., et al. (2017). Ribosome rearrangements at the onset of translational bypassing. Science Advances, 3(6): e1700147. doi:10.1126/sciadv.1700147.

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Agirrezabala, X., Author
Samatova, E. N.1, Author              
Klimova, M.1, Author              
Zamora, M., Author
Gil-Carton, D., Author
Rodnina, M. V.1, Author              
Valle, M., Author
Affiliations:
1Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578598              

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Free keywords: bypassing; recoding; ribosome; structure; translation
 Abstract: Bypassing is a recoding event that leads to the translation of two distal open reading frames into a single polypeptide chain. We present the structure of a translating ribosome stalled at the bypassing take-off site of gene 60 of bacteriophage T4. The nascent peptide in the exit tunnel anchors the P-site peptidyl-tRNAGly to the ribosome and locks an inactive conformation of the peptidyl transferase center (PTC). The mRNA forms a short dynamic hairpin in the decoding site. The ribosomal subunits adopt a rolling conformation in which the rotation of the small subunit around its long axis causes the opening of the A-site region. Together, PTC conformation and mRNA structure safeguard against premature termination and read-through of the stop codon and reconfigure the ribosome to a state poised for take-off and sliding along the noncoding mRNA gap.

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Language(s): eng - English
 Dates: 2017-06-07
 Publication Status: Published online
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/sciadv.1700147
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Title: Science Advances
Source Genre: Journal
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Pages: 8 Volume / Issue: 3 (6) Sequence Number: e1700147 Start / End Page: - Identifier: -