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  Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source

Reddy, H. K., Yoon, C. H., Aquila, A., Awel, S., Ayyer, K., Barty, A., et al. (2017). Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. Scientific Data, 4: 170079. doi:10.1038/sdata.2017.79.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-93CE-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-93CF-4
Genre: Journal Article

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© Hemanth K.N. Reddy et al.
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https://doi.org/10.1038/sdata.2017.79 (Publisher version)
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 Creators:
Reddy, Hemanth K.N.1, Author
Yoon, Chun Hong2, Author
Aquila, Andrew2, Author
Awel, Salah3, 4, Author
Ayyer, Kartik3, Author
Barty, Anton3, Author
Berntsen, Peter5, Author
Bielecki, Johan1, Author
Bobkov, Sergey6, Author
Bucher, Maximilian2, 7, 8, Author
Carini, Gabriella A.2, Author
Carron, Sebastian2, Author
Chapman, Henry3, 4, 9, Author
Daurer, Benedikt1, Author
DeMirci, Hasan2, 10, Author
Ekeberg, Tomas1, Author
Fromme, Petra11, 12, Author
Hajdu, Janos1, 13, Author
Hanke, Max Felix1, Author
Hart, Philip2, Author
Hogue, Brenda G.11, 14, 15, AuthorHosseinizadeh, Ahmad16, AuthorKim, Yoonhee17, AuthorKirian, Richard A.18, AuthorKurta, Ruslan P.19, AuthorLarsson, Daniel S.D.1, AuthorLoh, N. Duane20, 21, AuthorMaia, Filipe R.N.C.1, AuthorMancuso, Adrian P.19, AuthorMühlig, Kerstin1, AuthorMunke, Anna1, AuthorNam, Daewoong17, AuthorNettelblad, Carl1, 22, AuthorOurmazd, Abbas16, AuthorRose, Max23, AuthorSchwander, Peter16, AuthorSeibert, Marvin1, AuthorSellberg, Jonas A.1, 24, AuthorSong, Changyong17, AuthorSpence, John C.H.18, AuthorSvenda, Martin1, AuthorVan der Schot, Gijs 1, AuthorVartanyants, Ivan A.23, 25, AuthorWilliams, Garth J.26, AuthorPaulraj, Lourdu Xavier3, 9, 27, 28, Author               more..
Affiliations:
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden, ou_persistent22              
2SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA, ou_persistent22              
3Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany, ou_persistent22              
4Centre for Ultrafast Imaging, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22              
5Australian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia, ou_persistent22              
6National Research Centre ‘Kurchatov Institute’, Akademika Kurchatova pl. 1, 123182 Moscow, Russia, ou_persistent22              
7Argonne National Laboratory, 9700 S. Cass Ave, Lemont, Illinois 60439, USA, ou_persistent22              
8Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstraße 36, 10623 Berlin, Germany, ou_persistent22              
9Department of Physics, University of Hamburg, Luruper Chausee 149, 22761 Hamburg, Germany, ou_persistent22              
10Stanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA, ou_persistent22              
11Biodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe, Arizona 85287-5001, USA, ou_persistent22              
12School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287-1604, USA, ou_persistent22              
13Institute of Physics AS CR, v.v.i., Na Slovance 2, 18221 Prague 8, Czech Republic, ou_persistent22              
14Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, Biodesign Institute at Arizona State University, Tempe, Arizona 85287, USA, ou_persistent22              
15Arizona State University, School of Life Sciences (SOLS), Tempe, Arizona 85287-5401, USA, ou_persistent22              
16Department of Physics, University of Wisconsin Milwaukee, KIRC, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA, ou_persistent22              
17Department of Physics, Pohang University of Science and Technology, Pohang 37673, Korea, ou_persistent22              
18Department of Physics, Arizona State University, Tempe, Arizona 85287, USA, ou_persistent22              
19European X-ray Free Electron Laser GmbH, Holzkoppel 4, 22869 Schenefeld, Germany, ou_persistent22              
20Department of Physics, National University of Singapore Blk S12, 2 Science Drive 3, Singapore 117551, ou_persistent22              
21Centre for Bio-imaging Sciences, National University of Singapore Blk S1A, 14 Science Drive 4, Singapore 117557, ou_persistent22              
22Division of Scientific Computing, Department of Information Technology, Science for Life Laboratory, Uppsala University, Husargatan 3 (Box 337), Uppsala SE-75105, Sweden, ou_persistent22              
23Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, D-22607 Hamburg, Germany, ou_persistent22              
24Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, Stockholm SE-106 91, Sweden, ou_persistent22              
25National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Kashirskoe shosse 31, 115409 Moscow, Russia, ou_persistent22              
26NSLS-II, Brookhaven National Laboratory, Upton, New York 11873, USA, ou_persistent22              
27International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
28Max-Planck Institute for the Structure and Dynamics of Matter, 22607 Hamburg, Germany, ou_persistent22              

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Free keywords: Biological physics, Single-molecule biophysics, Virus structures, X-ray tomography
 Abstract: Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65–70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.

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Language(s): eng - English
 Dates: 2017-01-092017-04-272017-06-272017-06-27
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/sdata.2017.79
 Degree: -

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Title: Scientific Data
  Abbreviation : Sci. Data
Source Genre: Journal
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Publ. Info: London, United Kingdom : Nature Publishing Group
Pages: - Volume / Issue: 4 Sequence Number: 170079 Start / End Page: - Identifier: Other: 2052-4463
CoNE: /journals/resource/2052-4463