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  MCPIP1 exogenous overexpression inhibits pathways regulating MYCN oncoprotein stability in neuroblastoma.

Boratyn, E., Nowak, I., Durbas, M., Horwacik, I., Sawicka, A., & Rokita, H. (2017). MCPIP1 exogenous overexpression inhibits pathways regulating MYCN oncoprotein stability in neuroblastoma. Journal of Cellular Biochemistry, 118(7), 1741-1755. doi:10.1002/jcb.25832.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-842B-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-842E-7
Genre: Journal Article

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 Creators:
Boratyn, E., Author
Nowak, I., Author
Durbas, M., Author
Horwacik, I., Author
Sawicka, A.1, Author              
Rokita, H., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: MCPIP1; RNase; NEUROBLASTOMA; MYCN; AKT/mTOR PATHWAY
 Abstract: The main physiological function of MCPIP1 (regnase-1) is negative regulation of inflammation. Moreover, roles of regnase-1 in apoptosis and differentiation have also been described, but its involvement in cancer is yet to be fully recognized. Earlier, we showed a lack of expression of MCPIP1 in both primary tumors and several neuroblastoma cell lines. Additionally, we reported that levels of MCPIP1 and the key neuroblastoma oncoprotein-MYCN were inversely correlated in BE(2)-C clones overexpressing the MCPIP1 gene. Here, we show that exogenous expression of the MCPIP1 protein decreases MYCN mRNA and protein levels without changing the MYCN mRNA half-life. Furthermore, it was shown that MCPIP1-wt exogenous expression affects levels and phosphorylation of MYCN partners such as Aurora A (Thr288), CDC2 (Tyr15 and Thr161), GSK3 beta (Ser9), and key cellular components of Akt/mTOR signaling, which regulate MYCN stability and activation. In accordance with the obtained results, we found increased phosphorylation of MYCN protein at Thr58 that causes destabilization of the oncoprotein. Moreover, it is shown that exogenous expression of MCPIP1 does not cause apoptosis. Our data extend knowledge on roles of MCPIP1 in our model and link the protein to regulation of expression and stability of MYCN through decrease of signaling via Akt/mTOR pathway.

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Language(s): eng - English
 Dates: 2017-03-272017-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/jcb.25832
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Title: Journal of Cellular Biochemistry
Source Genre: Journal
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Pages: - Volume / Issue: 118 (7) Sequence Number: - Start / End Page: 1741 - 1755 Identifier: -