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  DNA synthesis in nucleotide-permeable Escherichia coli cells: V. Structure of newly synthesized φX174 replicative form DNA

Geider, K., Lechner, H., & Hoffmann-Berling, H. (1972). DNA synthesis in nucleotide-permeable Escherichia coli cells: V. Structure of newly synthesized φX174 replicative form DNA. Journal of Molecular Biology (London), 69(3), 333-347. doi:10.1016/0022-2836(72)90248-3.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-926B-E Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-926C-C
Genre: Journal Article

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 Creators:
Geider, Klaus1, Author           
Lechner, Helga2, Author           
Hoffmann-Berling, Hartmut3, Author           
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
3Department of Molecular Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497702              

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 Abstract: Cells in the double-stranded replicative form DNA multiplication stage of the infection with bacteriophage φX174 were made permeable to nucleotides and the flow of dBrUTP density label into the RF‡ of the cells was followed. It is concluded that there is a major process of RF synthesis in the permeable cells which gives rise to half-synthetic RF (one new, one conserved strand) and a minor process which gives rise to RF in which the fraction of newly synthesized DNA is only about 5%. The rate of chain elongation in the major process is approximately 200 nucleotides/sec at 25 °C and there is infectivity connected with the newly synthesized strands. RF with new complementary strand accumulates more rapidly than RF with new viral strand during the early synthesis. In order to explain this and other results, it is proposed that intermediates of in vivo replication continue to replicate in the permeable cells and that the viral strand but not the complementary strand is initiated in some of these in vivo intermediates. The opposite is not thought to occur. 5% synthetic RF is thought to result from the filling of natural gaps in RFII synthesized in vivo. This RF is made both in polA− cells and in polA+ cells and is labelled at the 3′-hydroxyl end of a DNA chain.

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Language(s): eng - English
 Dates: 1971-08-122004-10-221972-08-28
 Publication Status: Issued
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0022-2836(72)90248-3
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Title: Journal of Molecular Biology (London)
  Other : J Mol Biol
Source Genre: Journal
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Publ. Info: London : Academic Press
Pages: - Volume / Issue: 69 (3) Sequence Number: - Start / End Page: 333 - 347 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/954922646042