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  Studies on the fluorescence of 1-anilino-8-naphthalenesulfonate by the membranes of the sarcoplasmic reticulum

Augustin, J., & Hasselbach, W. (1973). Studies on the fluorescence of 1-anilino-8-naphthalenesulfonate by the membranes of the sarcoplasmic reticulum. European Journal of Biochemistry, 35(1), 114-121. doi:10.1111/j.1432-1033.1973.tb02816.x.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-96C8-F Version Permalink: https://hdl.handle.net/21.11116/0000-000A-376E-E
Genre: Journal Article

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Augustin, Jan1, Author           
Hasselbach, Wilhelm2, Author           
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1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: 1The quantum yield of 1-anilino-8-naphthalenesulfonate associated with native and lipid-depleted sarcoplasmic vesicles is nearly identical. The slight increase of the average apparent dissociation constant of the interaction of this dye with lipid-depleted vesicles is accompanied by a decrease in the number of binding sites for the dye.
2Oleic acid is a noncompetitive inhibitor of the anilino-naphthalenesulfonate binding to native and lipid-depleted sarcoplasmic vesicles.
3Rising temperatures decrease the quantum yield and increase the average apparent dissociation constant.
4The polarisation of the fluorescence of membrane-bound anilino-naphthalenesulfonate reaches relatively high values with a significant increment for lipid-depleted sarcoplasmic vesicles. There is only a slight temperature dependence and no viscosity dependence for both native and lipid-depleted vesicles. The polarisation is reduced by rising concentrations of anilino-naphthalenesulfonate.
5Energy transfer measurements indicate that the dye is located very closely to the membrane-bound tryptophan, suggesting a donor-acceptor distance of 2 nm.

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Language(s): eng - English
 Dates: 1972-12-041973-02-192005-03-031973-05
 Publication Status: Issued
 Pages: 8
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 Rev. Type: Peer
 Identifiers: DOI: 10.1111/j.1432-1033.1973.tb02816.x
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Title: European Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 35 (1) Sequence Number: - Start / End Page: 114 - 121 Identifier: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040