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  Cryo-EM reconstruction of the Cafeteria roenbergensis virus capsid suggests novel assembly pathway for giant viruses

Xiao, C., Fischer, M., Bolotaulo, D. M., Ulloa-Rondeau, N., Avila, G. A., & Suttle, C. A. (2017). Cryo-EM reconstruction of the Cafeteria roenbergensis virus capsid suggests novel assembly pathway for giant viruses. Scientific Reports, 7: 5484, pp. 1-7. doi:10.1038/s41598-017-05824-w.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-9A0A-C Version Permalink: http://hdl.handle.net/21.11116/0000-0000-28A5-9
Genre: Journal Article

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Xiao, Chuan, Author
Fischer, Matthias1, Author              
Bolotaulo, Duer M., Author
Ulloa-Rondeau, Nancy, Author
Avila, Gustavo A., Author
Suttle, Curtis A., Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Whereas the protein composition and overall shape of several giant virus capsids have been described, the mechanism by which these large capsids assemble remains enigmatic. Here, we present a reconstruction of the capsid of Cafeteria roenbergensis virus (CroV), one of the largest viruses analyzed by cryo-electron microscopy (cryo-EM) to date. The CroV capsid has a diameter of 3,000 Å and a Triangulation number of 499. Unlike related mimiviruses, the CroV capsid is not decorated with glycosylated surface fibers, but features 30 Å-long surface protrusions that are formed by loops of the major capsid protein. Based on the orientation of capsomers in the cryo-EM reconstruction, we propose that the capsids of CroV and related giant viruses are assembled by a newly conceived assembly pathway that initiates at a five-fold vertex and continuously proceeds outwards in a spiraling fashion.

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Language(s): eng - English
 Dates: 2017-04-262017-06-022017-07-142017
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41598-017-05824-w
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 5484 Start / End Page: 1 - 7 Identifier: ISSN: 2045-2322
CoNE: /journals/resource/2045-2322