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  Whole-brain serial-section electron microscopy in larval zebrafish

Hildebrand, D. G. C., Cicconet, M., Iguel Torres, R. M., Choi, W., Quan, T. M., Moon, J., et al. (2017). Whole-brain serial-section electron microscopy in larval zebrafish. Nature, 545(7654), 345-349. doi:10.1038/nature22356.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-A50D-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-A50E-9
Genre: Journal Article

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 Creators:
Hildebrand, David Grant Colburn1, Author
Cicconet, Marcelo1, Author
Iguel Torres, Russel M.1, Author
Choi, Woohyuk1, Author
Quan, Tran Minh1, Author
Moon, Jungmin1, Author
Wetzel, Arthur Willis1, Author
Champion, Andrew Scott1, Author
Graham, Brett Jesse1, Author
Randlett, Owen1, Author
Plummer, George Scott1, Author
Portugues, Ruben2, Author              
Bianco, Isaac Henry1, Author
Saalfeld, Stephan1, Author
Baden, Alexander David1, Author
Lillaney, Kunal1, Author
Burns, Randal1, Author
Vogelstein, Joshua Tzvi1, Author
Schier, Alexander Franz1, Author
Lee, Wei-Chung Allen1, Author
Jeong, Won-Ki1, AuthorLichtman, Jeff William1, AuthorEngert, Florian1, Author more..
Affiliations:
1external, ou_persistent22              
2Max Planck Research Group: Sensorimotor Control / Portugues, MPI of Neurobiology, Max Planck Society, ou_2054291              

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Free keywords: CAENORHABDITIS-ELEGANS; CIRCUIT RECONSTRUCTION; SYNAPTIC ORGANIZATION; NEURAL CIRCUITS; HIGH-RESOLUTION; VISUAL-CORTEX; NETWORK; VOLUME; NEURONS; CONNECTIVITYScience & Technology - Other Topics;
 Abstract: High-resolution serial-section electron microscopy (ssEM) makes it possible to investigate the dense meshwork of axons, dendrites, and synapses that form neuronal circuits(1). However, the imaging scale required to comprehensively reconstruct these structures is more than ten orders of magnitude smaller than the spatial extents occupied by networks of interconnected neurons(2), some of which span nearly the entire brain. Difficulties in generating and handling data for large volumes at nanoscale resolution have thus restricted vertebrate studies to fragments of circuits. These efforts were recently transformed by advances in computing, sample handling, and imaging techniques(1), but high-resolution examination of entire brains remains a challenge. Here, we present ssEM data for the complete brain of a larval zebrafish (Danio rerio) at 5.5 days post-fertilization. Our approach utilizes multiple rounds of targeted imaging at different scales to reduce acquisition time and data management requirements. The resulting dataset can be analysed to reconstruct neuronal processes, permitting us to survey all myelinated axons (the projectome). These reconstructions enable precise investigations of neuronal morphology, which reveal remarkable bilateral symmetry in myelinated reticulospinal and lateral line afferent axons. We further set the stage for whole-brain structure-function comparisons by co-registering functional reference atlases and in vivo two-photon fluorescence microscopy data from the same specimen. All obtained images and reconstructions are provided as an open-access resource.

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Language(s): eng - English
 Dates: 2017-05-102017-05-18
 Publication Status: Published in print
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000401466500062
DOI: 10.1038/nature22356
 Degree: -

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Title: Nature
  Abbreviation : Nature
Source Genre: Journal
 Creator(s):
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 545 (7654) Sequence Number: - Start / End Page: 345 - 349 Identifier: ISSN: 0028-0836
CoNE: /journals/resource/954925427238