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  Intrinsic Circuits in the Lateral Central Amygdala

Hunt, S., Sun, Y., Kucukdereli, H., Klein, R., & Sah, P. (2017). Intrinsic Circuits in the Lateral Central Amygdala. eNeuro, 4(1): UNSP e0367-16.2017. doi:10.1523/ENEURO.0367-16.2017.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-BE86-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-BE87-C
Genre: Journal Article

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ENEURO.0367-16.2017.full.pdf (Publisher version), 4MB
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© 2017 Hunt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International.
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 Creators:
Hunt, Sarah1, Author
Sun, Yajie1, Author
Kucukdereli, Hakan2, Author              
Klein, Rüdiger2, Author              
Sah, Pankaj1, Author
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1external, ou_persistent22              
2Department: Molecules-Signaling-Development / Klein, MPI of Neurobiology, Max Planck Society, ou_1113546              

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Free keywords: CENTRAL NUCLEUS; PHYSIOLOGICAL-PROPERTIES; CONDITIONED FEAR; LEARNED FEAR; NEURONS; RAT; COMPLEX; INTERNEURONS; CONNECTIONS; DISSECTIONNeurosciences & Neurology; anxiety; emotion; fear; learning;
 Abstract: Network activity in the lateral central amygdala (CeL) plays a crucial role in fear learning and emotional processing. However, the local circuits of the CeL are not fully understood and have only recently begun to be explored in detail. Here, we characterized the intrinsic circuits in the CeL using paired whole-call patch-clamp recordings, immunohistochemistry, and optogenetics in C57BL/6J wild-type and somatostatin-cre (SOM-Cre) mice. Our results revealed that throughout the rostrocaudal extent of the CeL, neurons form inhibitory connections at a rate of similar to 29% with an average amplitude of 20 +/- 3 pA (at -40 mV). Inhibitory input from a single neuron is sufficient to halt firing in the postsynaptic neuron. Post hoc immunostaining for protein kinase C delta(PKC delta) in wild-type mice and paired recordings in SOM-Cre mice demonstrated that the most common local connections were PKC delta(-) -> PKC delta(+) and SOM(+) -> SOM(+). Finally, by optogenetically activating either SOM(+) or SOM(+) neurons, we found that almost all neurons in the CeL were innervated by these neuronal populations and that connections between like neurons were stronger than those between different neuronal types. These findings reveal a complex network of connections within the CeL and provide the foundations for future behavior-specific circuit analysis of this complex network.

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Language(s): eng - English
 Dates: 2017-02-23
 Publication Status: Published online
 Pages: 18
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Title: eNeuro
Source Genre: Journal
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Publ. Info: Washington, DC : Society for Neuroscience
Pages: - Volume / Issue: 4 (1) Sequence Number: UNSP e0367-16.2017 Start / End Page: - Identifier: CoNE: /journals/resource/106249492X