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  Gephyrin interacts with dynein light chains 1 and 2, components of motor protein complexes

Fuhrmann, J. C., Kins, S., Rostaing, P., El Far, O., Kirsch, J., Sheng, M., et al. (2002). Gephyrin interacts with dynein light chains 1 and 2, components of motor protein complexes. Journal of Neuroscience, 22(13), 5393-5402.

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 Creators:
Fuhrmann, J. C., Author
Kins, S.1, Author           
Rostaing, P., Author
El Far, O.1, Author           
Kirsch, J.1, Author           
Sheng, M., Author
Triller, A., Author
Betz, H.1, Author           
Kneussel, M.1, Author           
Affiliations:
1Neurochemistry Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461704              

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Free keywords: gephyrin; cytoplasmic dynein; myosin-Va; Dlc-1; PIN; LC8; Dlc- 2; inhibitory synapse; motor protein
 Abstract: The clustering of glycine receptors and major subtypes of GABA(A) receptors at inhibitory synapses is mediated by the tubulin-binding protein gephyrin. In an attempt to identify additional components of inhibitory postsynaptic specializations, we performed a yeast two-hybrid screen using gephyrin as bait. Multiple positive clones encoded either the dynein light chain-1 (Dlc-1), also known as dynein LC8 and protein inhibitor of neuronal nitric oxide synthase, or its homolog Dlc-2. Dlc-1 protein bound efficiently to gephyrin in in vitro binding assays and colocalized with gephyrin during coexpression in HEK293 cells. The binding site for Dlc was mapped to a fragment of 63 amino acids within the central linker domain of gephyrin. In hippocampal neurons, endogenous Dlc protein was enriched at synaptic sites identified by synaptophysin and gephyrin immunostaining. Immunoelectron microscopy in spinal cord sections revealed Dlc immunoreactivity at the edges of postsynaptic differentiations, in close contact with cytoskeletal structures and at the periphery of the Golgi apparatus. Because Dlc-1 and Dlc-2 have been described as stoichiometric components of cytoplasmic dynein and myosin-Va complexes, our results suggest that motor proteins are involved in the subcellular localization of gephyrin.

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Language(s): eng - English
 Dates: 2002
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 10514
ISI: 000176599100019
 Degree: -

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Title: Journal of Neuroscience
  Alternative Title : J. Neurosci.
Source Genre: Journal
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Pages: - Volume / Issue: 22 (13) Sequence Number: - Start / End Page: 5393 - 5402 Identifier: ISSN: 0270-6474