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  Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools

Foerster, D., Arnold-Ammer, I., Laurell, E., Barker, A. J., Fernandes, A. M., Finger-Baier, K., et al. (2017). Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools. Scientific Reports, 7: 5230. doi:10.1038/s41598-017-04657-x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-BEE6-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-BEE7-3
Genre: Journal Article

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 Creators:
Foerster, Dominique1, Author              
Arnold-Ammer, Irene1, Author              
Laurell, Eva1, Author              
Barker, Alison J.1, Author              
Fernandes, António M.1, Author              
Finger-Baier, Karin1, Author              
Filosa, Alessandro2, Author
Helmbrecht, Thomas O.1, Author              
Koelsc, Yvonne2, Author
Kuehn, Enrico1, Author              
Robles, Estuardo1, Author              
Slanchev, Krasimir1, Author              
Thiele, Tod R.1, Author              
Baier, Herwig1, Author              
Kubo, Fumi1, Author              
Affiliations:
1Department: Genes-Circuits-Behavior / Baier, MPI of Neurobiology, Max Planck Society, ou_1128545              
2external, ou_persistent22              

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Free keywords: ENHANCER-TRAP LINES; LARVAL ZEBRAFISH; NERVOUS-SYSTEM; OPTIC TECTUM; KNOCK-IN; EXPRESSION; GAL4; ARCHITECTURE; INTEGRATION; BEHAVIORScience & Technology - Other Topics;
 Abstract: Genetic access to small, reproducible sets of neurons is key to an understanding of the functional wiring of the brain. Here we report the generation of a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neurotransmitter-synthesizing enzymes and neuropeptides, were selected according to their expression patterns in small and unique subsets of neurons from diverse brain regions. BAC recombineering, followed by Tol2 transgenesis, was used to generate driver lines that label neuronal populations in patterns that, to a large but variable extent, recapitulate the endogenous gene expression. We used image registration to characterize, compare, and digitally superimpose the labeling patterns from our newly generated transgenic lines. This analysis revealed highly restricted and mutually exclusive tissue distributions, with striking resolution of layered brain regions such as the tectum or the rhombencephalon. We further show that a combination of Gal4 and Cre transgenes allows intersectional expression of a fluorescent reporter in regions where the expression of the two drivers overlaps. Taken together, our study offers new tools for functional studies of specific neural circuits in zebrafish.

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Language(s): eng - English
 Dates: 2017-07-12
 Publication Status: Published online
 Pages: 11
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 Table of Contents: -
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 5230 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: /journals/resource/2045-2322