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  Bioluminescent antibodies for point-of-care diagnostics

Xue, L., Yu, Q., Griss, R., Schena, A., & Johnsson, K. (2017). Bioluminescent antibodies for point-of-care diagnostics. Angewandte Chemie International Edition, 56(25), 7112-7116. doi:10.1002/anie.201702403.

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 Creators:
Xue , Lin, Author
Yu , Qiuliyang, Author
Griss , Rudolf, Author
Schena , Alberto, Author
Johnsson, Kai1, Author              
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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Free keywords: antibodies; bioluminescence; point-of-care monitoring; sensors; therapeutic drug monitoring
 Abstract: We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the latter being labeled with a synthetic fluorescent competitor of the antigen. Binding of the antigen, here synthetic drugs, by the sensor displaces the tethered fluorescent competitor from the antibody and disrupts bioluminescent resonance energy transfer (BRET) between the luciferase and fluorophore. The semisynthetic sensors display a tunable response range (submicromolar to submillimolar) and large dynamic range (ΔRmax >500 %), and they permit the quantification of analytes through spotting of the samples onto paper followed by analysis with a digital camera.

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Language(s): eng - English
 Dates: 2017-03-032017-04-112017-06-062017-06-12
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Angewandte Chemie International Edition
  Other : Angew. Chem., Int. Ed.
  Other : Angew. Chem. Int. Ed.
  Other : Angewandte Chemie, International Edition
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 56 (25) Sequence Number: - Start / End Page: 7112 - 7116 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851