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  Deciphering the molecular architecture of membrane contact sites by cryo-electron tomography

Collado, J., & Fernandez-Busnadiego, R. (2017). Deciphering the molecular architecture of membrane contact sites by cryo-electron tomography. SI, 1864(9), 1507-1512. doi:10.1016/j.bbamcr.2017.03.009.

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 Creators:
Collado, Javier1, Author              
Fernandez-Busnadiego, Ruben1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: MEAN FREE-PATH; ENDOPLASMIC-RETICULUM; EXTENDED SYNAPTOTAGMINS; IN-SITU; ELECTRON-MICROSCOPY; ORP/OSH PROTEINS; SKELETAL-MUSCLE; LIPID TRANSFER; STIM PROTEINS; ERBiochemistry & Molecular Biology; Cell Biology; Cryo-EM; Cryo-focused ion beam milling; Vitrification; Endoplasmic reticulum; Extended synaptotagmin; Membrane tether;
 Abstract: At membrane contact sites (MCS) two cellular membranes form tight appositions that play critical roles in fundamental phenomena such as lipid metabolism or Ca2+ homeostasis. The interest for these structures has surged in recent years, bringing about the characterization of a plethora of MCS-resident molecules. How those molecules are structurally organized at MCS remains enigmatic, limiting our understanding of MCS function. Whereas such molecular detail is obscured by conventional electron microscopy sample preparation, cryo-electron tomography (cryo-ET) allows high resolution imaging of cellular landscapes in close-to-native conditions. Here we briefly review the fundamentals of cryo-ET and how recent developments in this technique are beginning to unveil the molecular architecture of MCS. This article is part of a Special Issue entitled: Membrane Contact Sites edited by Christian Ungermann and Benoit Kornmann.

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Language(s): eng - English
 Dates: 2017-03-192017
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Project name : Grant FP7 GA ERC-2012-SyG_318987–ToPAG
Grant ID : 318987
Funding program : Funding Programme 7 (FP7)
Funding organization : European Commission (EC)

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Title: SI
Source Genre: Issue
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Publ. Info: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS : ELSEVIER SCIENCE BV
Pages: - Volume / Issue: 1864 (9) Sequence Number: - Start / End Page: 1507 - 1512 Identifier: ISSN: 0167-4889

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Title: Biochimica et Biophysica Acta-Molecular Cell Research
Source Genre: Journal
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Publ. Info: New York, NY : Elsevier
Pages: - Volume / Issue: 1864 Sequence Number: - Start / End Page: - Identifier: ISSN: 0167-4889
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702_4