English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes

Vindry, C., Marnef, A., Broomhead, H., Twyffels, L., Ozgur, S., Stoecklin, G., et al. (2017). Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes. Cell Reports, 20(5), 1187-1200. doi:10.1016/j.celrep.2017.06.091.

Item is

Files

show Files
hide Files
:
1-s2.0-S2211124717309415-main.pdf (Publisher version), 5MB
Name:
1-s2.0-S2211124717309415-main.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© 2017 The Author(s). Open Access funded by Biotechnology and Biological Sciences Research Council Under a Creative Commons license
License:
-

Locators

show
hide
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Vindry, Caroline1, Author
Marnef, Aline1, Author
Broomhead, Helen1, Author
Twyffels, Laure1, Author
Ozgur, Sevim2, Author           
Stoecklin, Georg1, Author
Llorian, Miriam1, Author
Smith, Christopher W.1, Author
Mata, Juan1, Author
Weil, Dominique1, Author
Standart, Nancy1, Author
Affiliations:
1external, ou_persistent22              
2Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

Content

show
hide
Free keywords: U4/U6.U5 TRI-SNRNP; SM-LIKE PROTEIN; MESSENGER-RNAS; CAJAL BODIES; TRANSLATIONAL REPRESSION; BODY FORMATION; DECAY FACTOR; YEAST; DEGRADATION; DEADENYLATIONCell Biology;
 Abstract: Pat1 RNA-binding proteins, enriched in processing bodies (P bodies), are key players in cytoplasmic 5' to 3' mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer. Using co-immunoprecipitation and immunofluorescence approaches coupled with RNAi, we provide evidence for a nuclear complex of Pat1b with the Lsm2-8 heptamer, which binds to the spliceosomal U6 small nuclear RNA (snRNA). Furthermore, we establish the set of interactions connecting Pat1b/Lsm2-8/U6 snRNA/SART3 and additional U4/U6. U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) components in Cajal bodies, the site of snRNP biogenesis. RNA sequencing following Pat1b depletion revealed the preferential upregulation of mRNAs normally found in P bodies and enriched in 3' UTR AU-rich elements. Changes in > 180 alternative splicing events were also observed, characterized by skipping of regulated exons with weak donor sites. Our data demonstrate the dual role of a decapping enhancer in pre-mRNA processing as well as in mRNA decay via distinct nuclear and cytoplasmic Lsm complexes.

Details

show
hide
Language(s): eng - English
 Dates: 2017-08-012017
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Elsevier
Pages: - Volume / Issue: 20 (5) Sequence Number: - Start / End Page: 1187 - 1200 Identifier: Other: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247