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  Multivalent binding of PWWP2A to H2A.Z regulates mitosis and neural crest differentiation

Puenzeler, S., Link, S., Wagner, G., Keilhauer, E. C., Kronbeck, N., Spitzer, R. M. M., et al. (2017). Multivalent binding of PWWP2A to H2A.Z regulates mitosis and neural crest differentiation. EMBO Journal, 36(15), 2263-2279. doi:10.15252/embj.201695757.

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 Creators:
Puenzeler, Sebastian1, Author
Link, Stephanie1, Author
Wagner, Gabriele1, Author
Keilhauer, Eva C.2, Author              
Kronbeck, Nina1, Author
Spitzer, Ramona M. M.1, Author
Leidescher, Susanne1, Author
Markaki, Yolanda1, Author
Mentele, Edith1, Author
Regnard, Catherine1, Author
Schneider, Katrin1, Author
Takahashi, Daisuke1, Author
Kusakabe, Masayuki1, Author
Vardabasso, Chiara1, Author
Zink, Lisa M.1, Author
Straub, Tobias1, Author
Bernstein, Emily1, Author
Harata, Masahiko1, Author
Leonhardt, Heinrich1, Author
Mann, Matthias2, Author              
Rupp, Ralph A. W.1, AuthorHake, Sandra B.1, Author more..
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: HISTONE VARIANT H2A.Z; CHROMATIN; XENOPUS; DNA; DOMAIN; GENE; HETEROCHROMATIN; NUCLEOSOMES; COMPLEXES; CHAPERONEBiochemistry & Molecular Biology; Cell Biology; chromatin; H2A.Z; mitosis; neural crest; PWWP2A;
 Abstract: Replacement of canonical histones with specialized histone variants promotes altering of chromatin structure and function. The essential histone variant H2A.Z affects various DNA-based processes via poorly understood mechanisms. Here, we determine the comprehensive interactome of H2A.Z and identify PWWP2A as a novel H2A.Z-nucleosome binder. PWWP2A is a functionally uncharacterized, vertebrate-specific protein that binds very tightly to chromatin through a concerted multivalent binding mode. Two internal protein regions mediate H2A.Z-specificity and nucleosome interaction, whereas the PWWP domain exhibits direct DNA binding. Genome-wide mapping reveals that PWWP2A binds selectively to H2A.Z-containing nucleosomes with strong preference for promoters of highly transcribed genes. In human cells, its depletion affects gene expression and impairs proliferation via a mitotic delay. While PWWP2A does not influence H2A.Z occupancy, the C-terminal tail of H2A.Z is one important mediator to recruit PWWP2A to chromatin. Knockdown of PWWP2A in Xenopus results in severe cranial facial defects, arising from neural crest cell differentiation and migration problems. Thus, PWWP2A is a novel H2A.Z-specific multivalent chromatin binder providing a surprising link between H2A.Z, chromosome segregation, and organ development.

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Language(s): eng - English
 Dates: 2017-06-232017-08-01
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000406670200009
DOI: 10.15252/embj.201695757
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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 36 (15) Sequence Number: - Start / End Page: 2263 - 2279 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061