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  The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin

Woodruff, J. B., Gomes, B. F., Widlund, P. O., Mahamid, J., Honigmann, A., & Hyman, A. A. (2017). The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin. Cell, 169(6), 1066-1077.e10. doi:10.1016/j.cell.2017.05.028.

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 Creators:
Woodruff, Jeffrey B.1, Author
Gomes, Beatriz Ferreira1, Author
Widlund, Per O.1, Author
Mahamid, Julia2, Author              
Honigmann, Alf1, Author
Hyman, Anthony A.1, Author
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1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: CAENORHABDITIS-ELEGANS EMBRYOS; GAMMA-TUBULIN; PERICENTRIOLAR MATERIAL; C. ELEGANS; IN-VITRO; ORGANIZING CENTER; MITOTIC SPINDLES; PROTEIN SPD-2; RING COMPLEX; AURORA-ABiochemistry & Molecular Biology; Cell Biology;
 Abstract: Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted PCM-dependent microtubule nucleation in vitro using recombinant C. elegans proteins. We found that macromolecular crowding drives assembly of the key PCM scaffold protein SPD-5 into spherical condensates that morphologically and dynamically resemble in vivo PCM. These SPD-5 condensates recruited the microtubule polymerase ZYG-9 (XMAP215 homolog) and the microtubule-stabilizing protein TPXL-1 (TPX2 homolog). Together, these three proteins concentrated tubulin similar to 4-fold over background, which was sufficient to reconstitute nucleation of microtubule asters in vitro. Our results suggest that in vivo PCM is a selective phase that organizes microtubule arrays through localized concentration of tubulin by microtubule effector proteins.

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Language(s): eng - English
 Dates: 2017-06-012017
 Publication Status: Published in print
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 169 (6) Sequence Number: - Start / End Page: 1066 - 1077.e10 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183