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  Cellular Prion Protein PrPC and Ecto-5 '-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy

Domingues, P. H., Nanduri, L. S. Y., Seget, K., Venkateswaran, S. V., Agorku, D., Vigano, C., et al. (2017). Cellular Prion Protein PrPC and Ecto-5 '-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy. Cancer research: an official organ of the American Association for Cancer Research, 77(11), 2914-2926. doi:10.1158/0008-5472.CAN-16-3052.

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 Creators:
Domingues, Patrcia H.1, Author
Nanduri, Lalitha S. Y.1, Author
Seget, Katarzyna2, Author              
Venkateswaran, Sharavan V.1, Author
Agorku, David1, Author
Vigano, Cristina1, Author
von Schubert, Conrad1, Author
Nigg, Erich A.1, Author
Swanton, Charles1, Author
Sotillo, Rocio1, Author
Bosio, Andreas1, Author
Storchova, Zuzana2, Author              
Hardt, Olaf1, Author
Affiliations:
1external, ou_persistent22              
2Storchova, Zuzana / Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565171              

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Free keywords: CHROMOSOMAL INSTABILITY; HUMAN-CELLS; INDUCED APOPTOSIS; OXIDATIVE STRESS; CANCER BIOLOGY; SURVIVAL; OVEREXPRESSION; YEAST; TUMORIGENESIS; OPPORTUNITIESOncology;
 Abstract: Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of approximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples, and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrPC and the immunosuppressive cell surface enzyme ecto-5'-nucleotidase CD73. Functional analyses associated these alterations with increased cellular stress. An increased number of CD73(+) cells was observed in confluent cultures in aneuploid cells relative to their diploid counterparts. An elevated expression in CD230/PrPC was observed in serum-deprived cells in association with increased generation of reactive oxygen species. Overall, our work identified biomarkers of aneuploid karyotypes, which suggest insights into the underlying molecular physiology of aneuploid cells. (C) 2017 AACR.

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Language(s): eng - English
 Dates: 2017-04-042017
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Cancer research : an official organ of the American Association for Cancer Research
  Other : Cancer Res.
Source Genre: Journal
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Publ. Info: Baltimore, Md. : Waverly Press
Pages: - Volume / Issue: 77 (11) Sequence Number: - Start / End Page: 2914 - 2926 Identifier: ISSN: 0008-5472
CoNE: https://pure.mpg.de/cone/journals/resource/991042743115962