ausblenden:
Schlagwörter:
PATENT DUCTUS-ARTERIOSUS; NEONATAL PLATELETS; GESTATIONAL-AGE;
P-SELECTIN; LEUKOCYTE RECRUITMENT; INTEGRIN ACTIVATION; THROMBUS
FORMATION; YOLK-SAC; HEMOSTASIS; MICEHematology; Cardiovascular System & Cardiology; blood platelets; fetal development; hemostasis; intravital microscopy;
microcirculation; thrombosis;
Zusammenfassung:
Objective-Platelet function has been intensively studied in the adult organism. However, little is known about the function and hemostatic capacity of platelets in the developing fetus as suitable in vivo models are lacking.
Approach and Results-To examine fetal platelet function in vivo, we generated a fetal thrombosis model and investigated light/dye-induced thrombus formation by intravital microscopy throughout gestation. We observed that significantly less and unstable thrombi were formed at embryonic day (E) 13.5 compared with E17.5. Flow cytometry revealed significantly lower platelet counts in E13.5 versus E17.5 fetuses versus adult controls. In addition, fetal platelets demonstrated changed activation responses of surface adhesion molecules and reduced P-selectin content and mobilization. Interestingly, we also measured reduced levels of the integrin-activating proteins Kindlin-3, Talin-1, and Rap1 during fetal development. Consistently, fetal platelets demonstrated diminished spreading capacity compared with adults. Transfusion of adult platelets into the fetal circulation led to rapid platelet aggregate formation even in young fetuses. Yet, retrospective data analysis of a neonatal cohort demonstrated no correlation of platelet transfusion with closure of a persistent ductus arteriosus, a process reported to be platelet dependent.
Conclusions-Taken together, we demonstrate an ontogenetic regulation of platelet function in vivo with physiologically low platelet numbers and hyporeactivity early during fetal development shedding new light on hemostatic function during fetal life.
