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  Structural basis of outer dynein arm intraflagellar transport by the transport adaptor protein ODA16 and the intraflagellar transport protein IFT46

Taschner, M., Mourao, A., Awasthi, M., Basquin, J., & Lorentzen, E. (2017). Structural basis of outer dynein arm intraflagellar transport by the transport adaptor protein ODA16 and the intraflagellar transport protein IFT46. Journal of Biological Chemistry, 292(18), 7462-7473. doi:10.1074/jbc.M117.780155.

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 Urheber:
Taschner, Michael1, Autor
Mourao, Andre1, Autor
Awasthi, Mayanka1, Autor
Basquin, Jerome2, Autor           
Lorentzen, Esben1, Autor
Affiliations:
1external, ou_persistent22              
2Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

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Schlagwörter: B CORE COMPLEX; SMALL-ANGLE SCATTERING; BIOLOGICAL MACROMOLECULES; CRYOELECTRON TOMOGRAPHY; TUBULIN TRANSPORT; CRYSTAL-STRUCTURE; MOTOR DOMAIN; ARCHITECTURE; FLAGELLA; CARGOBiochemistry & Molecular Biology; adaptor protein; crystal structure; dynein; intracellular trafficking; primary cilium; IFT; cilium; flagellum; intraflagellar transport; outer dynein arms;
 Zusammenfassung: Motile cilia are found on unicellular organisms such as the green alga Chlamydomonas reinhardtii, on sperm cells, and on cells that line the trachea and fallopian tubes in mammals. The motility of cilia relies on a number of large protein complexes including the force-generating outer dynein arms (ODAs). The transport of ODAs into cilia has been previously shown to require the transport adaptor ODA16, as well as the intraflagellar transport (IFT) protein IFT46, but the molecular mechanism by which ODAs are recognized and transported into motile cilia is still unclear. Here, we determined the high-resolution crystal structure of C. reinhardtii ODA16 (CrODA16) and mapped the binding to IFT46 and ODAs. The CrODA16 structure revealed a small 80-residue N-terminal domain and a C-terminal 8-bladed -propeller domain that are both required for the association with the N-terminal 147 residues of IFT46. The dissociation constant of the IFT46-ODA16 complex was 200 nm, demonstrating that CrODA16 associates with the IFT complex with an affinity comparable with that of the individual IFT subunits. Furthermore, we show, using ODAs extracted from the axonemes of C. reinhardtii, that the C-terminal -propeller but not the N-terminal domain of CrODA16 is required for the interaction with ODAs. These data allowed us to present an architectural model for ODA16-mediated IFT of ODAs.

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Sprache(n): eng - English
 Datum: 2017
 Publikationsstatus: Erschienen
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000400761300016
DOI: 10.1074/jbc.M117.780155
 Art des Abschluß: -

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Titel: Journal of Biological Chemistry
  Andere : J. Biol. Chem.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Seiten: - Band / Heft: 292 (18) Artikelnummer: - Start- / Endseite: 7462 - 7473 Identifikator: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826