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  Expanding the boundaries of cryo-EM with phase plates.

Danev, R., & Baumeister, W. (2017). Expanding the boundaries of cryo-EM with phase plates. Current opinion in structural biology, 46, 87-94. doi:10.1016/j.sbi.2017.06.006.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-EEC3-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-EEC4-5
Genre: Journal Article

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 Creators:
Danev, Radostin1, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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 Abstract: Phase plates have long been considered as a means for improving the performance of cryo-electron microscopy (cryo-EM). But practical limitations, such as a short lifespan or cumbersome usage have prevented their widespread adoption. The recently developed Volta phase plate overcomes most of the practical issues and it is now commercially available. Results from both, electron cryo-tomography (cryo-ET) and single particle analysis (SPA), have demonstrated the benefits of using a phase plate. In CET phase plates have helped to visualize cellular ultrastructure in unprecedented detail. In SPA phase plates allowed to determine the structures of small proteins at near-atomic resolutions. Further improvements in phase plate technology are possible and new designs are already under development. Copyright 2017 Elsevier Ltd. All rights reserved.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: ISI: 28675816
DOI: 10.1016/j.sbi.2017.06.006
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Title: Current opinion in structural biology
Source Genre: Journal
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Pages: - Volume / Issue: 46 Sequence Number: - Start / End Page: 87 - 94 Identifier: ISSN: 1879-033X