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  Proteome Analysis of Human Follicular Thyroid Cancer Cells Exposed to the Random Positioning Machine.

Bauer, J., Kopp, S., Schlagberger, E. M., Grosse, J., Sahana, J., Riwaldt, S., et al. (2017). Proteome Analysis of Human Follicular Thyroid Cancer Cells Exposed to the Random Positioning Machine. International Journal of Molecular Sciences, 18(3): 546. doi:10.3390/ijms18030546.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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 Creators:
Bauer, Johann1, Author              
Kopp, Sascha2, Author
Schlagberger, Elisabeth Maria1, Author              
Grosse, Jirka2, Author
Sahana, Jayashree2, Author
Riwaldt, Stefan2, Author
Wehland, Markus2, Author
Luetzenberg, Ronald2, Author
Infanger, Manfred2, Author
Grimm, Daniela2, Author
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1Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              
2external, ou_persistent22              

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Free keywords: cellular compartments; mass spectrometry; pathway analysis; proteomics; random positioning machineOncology; Cell Biology; Chemistry; Biochemistry & Molecular Biology; Genetics & Heredity; Physics (provided by Clarivate Analytics);
 Abstract: Several years ago, we detected the formation of multicellular spheroids in experiments with human thyroid cancer cells cultured on the Random Positioning Machine (RPM), a ground-based model to simulate microgravity by continuously changing the orientation of samples. Since then, we have studied cellular mechanisms triggering the cells to leave a monolayer and aggregate to spheroids. Our work focused on spheroid-related changes in gene expression patterns, in protein concentrations, and in factors secreted to the culture supernatant during the period when growth is altered. We detected that factors inducing angiogenesis, the composition of integrins, the density of the cell monolayer exposed to microgravity, the enhanced production of caveolin-1, and the nuclear factor kappa B p65 could play a role during spheroid formation in thyroid cancer cells. In this study, we performed a deep proteome analysis on FTC-133 thyroid cancer cells cultured under conditions designed to encourage or discourage spheroid formation. The experiments revealed more than 5900 proteins. Their evaluation confirmed and explained the observations mentioned above. In addition, we learned that FTC-133 cells growing in monolayers or in spheroids after RPM-exposure incorporate vinculin, paxillin, focal adhesion kinase 1, and adenine diphosphate (ADP)-ribosylation factor 6 in different ways into the focal adhesion complex.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 28273809
DOI: 10.3390/ijms18030546
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Title: International Journal of Molecular Sciences
  Abbreviation : Int. J. Mol. Sci.
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 18 (3) Sequence Number: 546 Start / End Page: - Identifier: ISSN: 1422-0067
CoNE: https://pure.mpg.de/cone/journals/resource/1422-0067