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  Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state

Snijder, J., Schuller, J. M., Wiegard, A., Lossl, P., Schmelling, N., Axmann, I. M., et al. (2017). Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state. Science, 355(6330), 1181-1184. doi:10.1126/science.aag3218.

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 Creators:
Snijder, Joost1, Author
Schuller, Jan M.2, Author              
Wiegard, Anika1, Author
Lossl, Philip1, Author
Schmelling, Nicolas1, Author
Axmann, Ilka M.1, Author
Plitzko, Jürgen M.2, Author              
Förster, Friedrich2, Author              
Heck, Albert J. R.1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: CLOCK PROTEIN KAIB; CRYSTAL-STRUCTURE; PHOSPHORYLATION; DYNAMICS; COMPLEX; KAIABC; RHYTHM; EXPRESSIONScience & Technology - Other Topics;
 Abstract: Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000396351200037
DOI: 10.1126/science.aag3218
 Degree: -

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Title: Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 355 (6330) Sequence Number: - Start / End Page: 1181 - 1184 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1