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  Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

Trussart, M., Yus, E., Martinez, S., Bau, D., Tahara, Y. O., Pengo, T., et al. (2017). Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae. Nature Communications, 8: 14665. doi:10.1038/ncomms14665.

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Trussart, Marie1, Author
Yus, Eva1, Author
Martinez, Sira1, Author
Bau, Davide1, Author
Tahara, Yuhei O.1, Author
Pengo, Thomas1, Author
Widjaja, Michael1, Author
Kretschmer, Simon2, Author              
Swoger, Jim1, Author
Djordjevic, Steven1, Author
Turnbull, Lynne1, Author
Whitchurch, Cynthia1, Author
Miyata, Makoto1, Author
Marti-Renom, Marc A.1, Author
Lluch-Senar, Maria1, Author
Serrano, Luis1, Author
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1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: ESCHERICHIA-COLI CHROMOSOME; SINGLE-STRANDED-DNA; GENE-REGULATION; CYTADHERENCE PROTEINS; ATTACHMENT ORGANELLE; SPATIAL-ORGANIZATION; TOPOLOGICAL DOMAINS; ELECTRON-MICROSCOPY; BACILLUS-SUBTILIS; CRYSTAL-STRUCTUREScience & Technology - Other Topics;
 Abstract: DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.

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Language(s): eng - English
 Dates: 2017-03-08
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000395678600001
DOI: 10.1038/ncomms14665
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 14665 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723