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  Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin

Bürmann, F., Basfeld, A., Nunez, R. V., Diebold-Durand, M.-L., Wilhelm, L., & Gruber, S. (2017). Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin. Molecular Cell, 65(5), 861-872.e9. doi:10.1016/j.molcel.2017.01.026.

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 Creators:
Bürmann, Frank1, Author              
Basfeld, Alrun1, Author              
Nunez, Roberto Vazquez2, Author
Diebold-Durand, Marie-Laure1, Author              
Wilhelm, Larissa1, Author              
Gruber, Stephan1, Author              
Affiliations:
1Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              
2external, ou_persistent22              

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Free keywords: COILED-COIL DOMAIN; BACILLUS-SUBTILIS; DNA-BINDING; BACTERIAL CHROMOSOME; MOLECULAR-BASIS; COHESIN RING; PROTEINS; ATPASE; SEGREGATION; HINGEBiochemistry & Molecular Biology; Cell Biology;
 Abstract: SMC proteins support vital cellular processes in all domains of life by organizing chromosomal DNA. They are composed of ATPase "head" and "hinge" dimerization domains and a connecting coiled-coil "arm." Binding to a kleisin subunit creates a closed tripartite ring, whose similar to 47-nm-long SMC arms act as barrier for DNA entrapment. Here, we uncover another, more active function of the bacterial Smc arm. Using high-throughput genetic engineering, we resized the arm in the range of 6-60 nm and found that it was functional only in specific length regimes following a periodic pattern. Natural SMC sequences reflect these length constraints. Mutants with improper arm length or peptide insertions in the arm efficiently target chromosomal loading sites and hydrolyze ATP but fail to use ATP hydrolysis for relocation onto flanking DNA. We propose that SMC arms implement force transmission upon nucleotide hydrolysis to mediate DNA capture or loop extrusion.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 65 (5) Sequence Number: - Start / End Page: 861 - 872.e9 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929