English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Two novel effectors of trafficking and maturation of the yeast plasma membrane H+-ATPase.

Geva, Y., Crissmann, J., Arakel, E. Z., Gómez-Navarro, N., Chuartzman, S. G., Stahmer, K. R., et al. (2017). Two novel effectors of trafficking and maturation of the yeast plasma membrane H+-ATPase. Traffic, 18(10), 672-682. doi:10.1111/tra.12503.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-D3A0-E Version Permalink: http://hdl.handle.net/21.11116/0000-0001-2239-9
Genre: Journal Article

Files

show Files
hide Files
:
2473473.pdf (Publisher version), 15MB
Name:
2473473.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2473473_Suppl_1.pdf (Supplementary material), 122KB
Name:
2473473_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2473473_Suppl_2.xlsx (Supplementary material), 11KB
Name:
2473473_Suppl_2.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2473473_Suppl_3.xlsx (Supplementary material), 11KB
Name:
2473473_Suppl_3.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2473473_Suppl_4.xlsx (Supplementary material), 20KB
Name:
2473473_Suppl_4.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2473473_Suppl_5.xlsx (Supplementary material), 9KB
Name:
2473473_Suppl_5.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2473473_Suppl_6.xlsx (Supplementary material), 17KB
Name:
2473473_Suppl_6.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2473473_Suppl_7.xlsx (Supplementary material), 16KB
Name:
2473473_Suppl_7.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2473473_Suppl_8.docx (Supplementary material), 2MB
Name:
2473473_Suppl_8.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Geva, Y., Author
Crissmann, J., Author
Arakel, E. Z., Author
Gómez-Navarro, N., Author
Chuartzman, S. G., Author
Stahmer, K. R., Author
Schwappach, B.1, Author              
Miller, E. A., Author
Schuldiner, M., Author
Affiliations:
1Max Planck Fellow Blanche Schwappach, ou_1548137              

Content

show
hide
Free keywords: -
 Abstract: The endoplasmic reticulum (ER) is the entry site of proteins into the endomembrane system. Proteins exit the ER via coat protein II (COPII) vesicles in a selective manner, mediated either by direct interaction with the COPII coat or aided by cargo receptors. Despite the fundamental role of such receptors in protein sorting, only a few have been identified. To further define the machinery that packages secretory cargo and targets proteins from the ER to Golgi membranes, we used multiple systematic approaches, which revealed 2 uncharacterized proteins that mediate the trafficking and maturation of Pma1, the essential yeast plasma membrane proton ATPase. Ydl121c (Exp1) is an ER protein that binds Pma1, is packaged into COPII vesicles, and whose deletion causes ER retention of Pma1. Ykl077w (Psg1) physically interacts with Exp1 and can be found in the Golgi and coat protein I (COPI) vesicles but does not directly bind Pma1. Loss of Psg1 causes enhanced degradation of Pma1 in the vacuole. Our findings suggest that Exp1 is a Pma1 cargo receptor and that Psg1 aids Pma1 maturation in the Golgi or affects its retrieval. More generally our work shows the utility of high content screens in the identification of novel trafficking components.

Details

show
hide
Language(s): eng - English
 Dates: 2017-08-162017-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1111/tra.12503
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Traffic
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 18 (10) Sequence Number: - Start / End Page: 672 - 682 Identifier: -