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Abstract:
Tomato bushy stunt virus (TBSV) and turnip crinkle virus (TCV) each contain two minor polypeptides in addition to the major coat protein. We have used quantitative estimation of band strengths after SDS-gel electrophoresis to show that the larger of these species (MW 87,000 in TBSV, 84,000 in TCV) is always present at a level of about one chain per particle. The smaller polypeptide (MW 28,000), present in variable amounts, is shown to be a cleavage product of the major coat protein (MW 41,000).