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  Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100(T) revealed by de novo genome project data

Toth, A., Baka, E., Luzics, S., Bata-Vidacs, I., Nagy, I., Balint, B., et al. (2017). Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100(T) revealed by de novo genome project data. Acta Alimentaria, 46(3), 323-335. doi:10.1556/066.2016.0014.

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This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/).
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 Creators:
Toth, A.1, Author
Baka, E.1, Author
Luzics, Sz.1, Author
Bata-Vidacs, I.1, Author
Nagy, I.1, Author
Balint, B.1, Author
Herczeg, R.1, Author
Olasz, F.1, Author
Wilk, T.1, Author
Nagy, T.1, Author
Kriszt, B.1, Author
Nagy, I.2, Author              
Kukolya, J.1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: THERMOMONOSPORA-FUSCA XYLANASE; PROTEIN FAMILIES DATABASE; STREPTOMYCES-LIVIDANS; BIOCHEMICAL-CHARACTERIZATION; CATALYTIC DOMAIN; ENDOGLUCANASE E2; BETA-MANNANASE; DNA-SEQUENCES; CLONING; GENEFood Science & Technology; Nutrition & Dietetics; de novo genome project; glycoside hydrolyses; Thermobifida; cellulases; hemicellulases; amylases;
 Abstract: Thermobifidas are thermophilic, aerobic, lignocellulose decomposing actinomycetes. The Thermobifida genus includes four species: T. fusca, T. alba, T. cellulosilytica, and T. halotolerans. T. fusca YX is the far best characterized strain of this taxon and several cellulases and hemicellulases have been cloned from it for industrial purposes targeting paper industry, biofuel, and feed applications. Unfortunately, sequence data of such enzymes are almost exclusively restricted to this single species; however, we demonstrated earlier by zymography that other T. alba and T. cellulosilytica strains encode the same enzyme sets. Recently, the advances in whole genome sequencing by the use of next generation genomics platforms accelerated the selection process of valuable hydrolases from uncharacterized bacterial species for cloning purposes. For this purpose T. cellulosilytica TB100(T) type strain was chosen for de novo genome sequencing. We have assembled the genome of T. cellulosilytica strain TB100(T) into 168 contigs and 19 scaffolds, with reference length of 4 327 869 bps, 3 589 putative coding sequences, 53 tRNAs, and 4 rRNAs. The analysis of the annotated genome revealed the existence of 27 putative hydrolases belonging to 14 different glycoside hydrolase (GH) families. The investigation of identified, cloned, and heterologously multiple cellulases, mannanases, xylanases, and amylases may result in industrial applications beside gaining useful basic research related information.

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Language(s): eng - English
 Dates: 2016-03-312017-09
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000407346900008
DOI: 10.1556/066.2016.0014
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Title: Acta Alimentaria
  Subtitle : An International Journal of Food Science
Source Genre: Journal
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Publ. Info: PRIELLE K U 19, PO BOX 245,, H-1117 BUDAPEST, HUNGARY : AKADEMIAI KIADO RT
Pages: - Volume / Issue: 46 (3) Sequence Number: - Start / End Page: 323 - 335 Identifier: ISSN: 0139-3006