English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Insulin-From its Discovery to the Industrial Synthesis of Modern Insulin Analogues

Moroder, L., & Musiol, H.-J. (2017). Insulin-From its Discovery to the Industrial Synthesis of Modern Insulin Analogues. Angewandte Chemie International Edition, 56(36), 10656-10669. doi:10.1002/anie.201702493.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Moroder, Luis1, Author           
Musiol, Hans-Jürgen1, Author           
Affiliations:
1Moroder, Luis / Bioorganic Chemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565160              

Content

show
hide
Free keywords: PHASE PEPTIDE-SYNTHESIS; ACETAMIDOMETHYL-CYSTEINE-PEPTIDES; CRYSTALLINE BOVINE INSULIN; DISULFIDE BOND FORMATION; CHAIN DES-(B30) INSULIN; ORGANIC DIVALENT SULFUR; AMINO-ACID-SEQUENCE; S-TRITYL-CYSTEINE; CHEMICAL-SYNTHESIS; B-CHAINChemistry; biosynthesis; insulin; native chemical ligation; semisynthesis; solid-phase synthesis;
 Abstract: After the discovery of insulin as a drug for diabetes, the pharmaceutical companies were faced with the challenge to meet the demand for insulin with the highest possible degree of purity in the required quantities from animal sources. The observation of an immune reaction of patients to insulin from animal pancreatic extracts made the availability of human insulin of highest priority. Only the enzyme-catalyzed semisynthesis at the C-terminus of the insulin B-chain led to a commercial process, but it depended on porcine insulin and was aggravated by supply concerns. The advent of rDNA technology allowed the commercial preparation of human insulin by biosynthesis in virtually unlimited quantities. An increased chemical diversity was only envisaged through chemical synthesis, which was simplified by advances in solid-phase peptide synthesis and chemical ligation. Single-chain insulin precursors are now being synthesized that should enable fast screening of insulin analogues for improved biophysical, biological, and thus promising new therapeutic properties, as well as for the industrial manufacture of insulin analogues not accessible by biosynthesis.

Details

show
hide
Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000408267000004
DOI: 10.1002/anie.201702493
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Angewandte Chemie International Edition
  Other : Angew. Chem., Int. Ed.
  Other : Angew. Chem. Int. Ed.
  Other : Angewandte Chemie, International Edition
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 56 (36) Sequence Number: - Start / End Page: 10656 - 10669 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851