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  Non-canonical binding site for bacterial initiation factor 3 on the large ribosomal subunit.

Goyal, A., Belardinelli, R., & Rodnina, M. V. (2017). Non-canonical binding site for bacterial initiation factor 3 on the large ribosomal subunit. Cell Reports, 20(13), 3113-3122. doi:10.1016/j.celrep.2017.09.012.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-E13C-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F8FD-5
Genre: Journal Article

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 Creators:
Goyal, A.1, Author              
Belardinelli, R.1, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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Free keywords: ribosome; bacterial translation initiation; IF3; 50S subunit; 70S initiation complex; electrostatic interactions; non-canonical initiation; leaderless initiation; re-initiation; IF3 dynamics
 Abstract: Canonical translation initiation in bacteria entails the assembly of the 30S initiation complex (IC), which binds the 50S subunit to form a 70S IC. IF3, a key initiation factor, is recruited to the 30S subunit at an early stage and is displaced from its primary binding site upon subunit joining. We employed four different FRET pairs to monitor IF3 relocation after 50S joining. IF3 moves away from the 30S subunit, IF1 and IF2, but can remain bound to the mature 70S IC. The secondary binding site is located on the 50S subunit in the vicinity of ribosomal protein L33. The interaction between IF3 and the 50S subunit is largely electrostatic with very high rates of IF3 binding and dissociation. The existence of the non-canonical binding site may help explain how IF3 participates in alternative initiation modes performed directly by the 70S ribosomes, such as initiation on leaderless mRNAs or re-initiation.

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Language(s): eng - English
 Dates: 2017-09-26
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.celrep.2017.09.012
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Title: Cell Reports
Source Genre: Journal
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Pages: - Volume / Issue: 20 (13) Sequence Number: - Start / End Page: 3113 - 3122 Identifier: -