English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Stable positioning of Unc13 restricts synaptic vesicle fusion to defined release sites to promote synchronous neurotransmission.

Reddy-Alla, S., Böhme, M. A., Reynolds, E., Beis, C., Grasskamp, A. T., Mampell, M. M., et al. (2017). Stable positioning of Unc13 restricts synaptic vesicle fusion to defined release sites to promote synchronous neurotransmission. Neuron, 95(6), 1350-1364. doi:10.1016/j.neuron.2017.08.016.

Item is

Files

show Files
hide Files
:
2477083.pdf (Publisher version), 6MB
 
File Permalink:
-
Name:
2477083.pdf
Description:
-
OA-Status:
Visibility:
Restricted (UNKNOWN id 303; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2477083_Suppl_1.pdf (Supplementary material), 2MB
Name:
2477083_Suppl_1.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2477083_Suppl_2.xlsx (Supplementary material), 84KB
Name:
2477083_Suppl_2.xlsx
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2477083_Suppl_3.mp4 (Supplementary material), 9MB
Name:
2477083_Suppl_3.mp4
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
video/mp4 / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2477083_Suppl_4.pdf (Supplementary material), 8MB
Name:
2477083_Suppl_4.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2477083.pdf (Publisher version), 6MB
 
File Permalink:
-
Name:
2477083.pdf
Description:
-
OA-Status:
Visibility:
Restricted (UNKNOWN id 303; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Reddy-Alla, S., Author
Böhme, M. A., Author
Reynolds, E., Author
Beis, C., Author
Grasskamp, A. T., Author
Mampell, M. M., Author
Maglione, M., Author
Jusyte, M., Author
Rey, U., Author
Babikir, H., Author
McCarthy, A. W., Author
Quentin, C., Author
Matkovic, T., Author
Bergeron, D. D., Author
Mushtaq, Z., Author
Göttfert, F.1, Author           
Owald, D., Author
Mielke, T., Author
Hell, S. W.1, Author           
Sigrist, S. J., Author
Walter, A. M., Author more..
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: synapse; Unc13; scaffolding proteins; active zone; release site; STED microscopy; local synaptic activity imaging; in vivo imaging; variance-mean analysis; high-pressure freeze electron microscopy
 Abstract: Neural information processing depends on precisely timed, Ca2+-activated synaptic vesicle exocytosis from release sites within active zones (AZs), but molecular details are unknown. Here, we identify that the (M)Unc13-family member Unc13A generates release sites and show the physiological relevance of their restrictive AZ targeting. Super-resolution and intravital imaging of Drosophila neuromuscular junctions revealed that (unlike the other release factors Unc18 and Syntaxin-1A) Unc13A was stably and precisely positioned at AZs. Local Unc13A levels predicted single AZ activity. Different Unc13A portions selectively affected release site number, position, and functionality. An N-terminal fragment stably localized to AZs, displaced endogenous Unc13A, and reduced the number of release sites, while a C-terminal fragment generated excessive sites at atypical locations, resulting in reduced and delayed evoked transmission that displayed excessive facilitation. Thus, release site generation by the Unc13A C terminus and their specific AZ localization via the N terminus ensure efficient transmission and prevent ectopic, temporally imprecise release.

Details

show
hide
Language(s): eng - English
 Dates: 2017-08-312017-09-13
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.neuron.2017.08.016
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Neuron
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 95 (6) Sequence Number: - Start / End Page: 1350 - 1364 Identifier: -