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  Solution NMR views of dynamical ordering of biomacromolecules.

Ikeya, T., Ban, D., Lee, D., Ito, Y., Kato, K., & Griesinger, C. (2018). Solution NMR views of dynamical ordering of biomacromolecules. Biochimica et Biophysica Acta - General Subjects, 1862(2), 287-306. doi:10.1016/j.bbagen.2017.08.020.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-E2AD-C Version Permalink: http://hdl.handle.net/21.11116/0000-0003-696F-C
Genre: Journal Article

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2477100.pdf (Publisher version), 3MB
 
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 Creators:
Ikeya, T., Author
Ban, D., Author
Lee, D., Author
Ito, Y., Author
Kato, K., Author
Griesinger, C.1, Author              
Affiliations:
1Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Free keywords: Dynamical ordering; Solution NMR spectroscopy; Dynamics; Large biomacromolecules; Stable isotope labelling; In-cell NMR
 Abstract: To understand the mechanisms related to the 'dynamical ordering' of macromolecules and biological systems, it is crucial to monitor, in detail, molecular interactions and their dynamics across multiple timescales. Solution nuclear magnetic resonance (NMR) spectroscopy is an ideal tool that can investigate biophysical events at the atomic level, in near-physiological buffer solutions, or even inside cells. Scope of Review In the past several decades, progress in solution NMR has significantly contributed to the elucidation of three-dimensional structures, the understanding of conformational motions, and the underlying thermodynamic and kinetic properties of biomacromolecules. This review discusses recent methodological development of NMR, their applications and some of the remaining challenges. Major Conclusions Although a major drawback of NMR is its difficulty in studying the dynamical ordering of larger biomolecular systems, current technologies have achieved considerable success in the structural analysis of substantially large proteins and biomolecular complexes over 1 MDa and have characterised a wide range of timescales across which biomolecular motion exists. While NMR is well suited to obtain local structure information in detail, it contributes valuable and unique information within hybrid approaches that combine complementary methodologies, including solution scattering and microscopic techniques. General Significance For living systems, the dynamic assembly and disassembly of macromolecular complexes is of utmost importance for cellular homeostasis and, if dysregulated, implied in human disease. It is thus instructive for the advancement of the study of the dynamical ordering to discuss the potential possibilities of solution NMR spectroscopy and its applications. This article is part of a Special Issue entitled "Biophysical Exploration of Dynamical Ordering of Biomolecular Systems" edited by Dr. Koichi Kato.

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Language(s): eng - English
 Dates: 2017-08-252018-02
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.bbagen.2017.08.020
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Title: Biochimica et Biophysica Acta - General Subjects
Source Genre: Journal
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Pages: - Volume / Issue: 1862 (2) Sequence Number: - Start / End Page: 287 - 306 Identifier: -