English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Calcium ions induce glutamate transport into rat brain membrane vesicles in the absence of sodium and chloride. Evidence for a novel uptake site?

Hollmann, M., Harnecker, J., & Seifert, W. (1988). Calcium ions induce glutamate transport into rat brain membrane vesicles in the absence of sodium and chloride. Evidence for a novel uptake site? FEBS Letters, 228(1), 74-78. doi:10.1016/0014-5793(88)80588-X.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-E967-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-E969-8
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hollmann, M.1, Author              
Harnecker, J.1, Author              
Seifert, W.1, Author              
Affiliations:
1Abteilung Neurobiologie, MPI for biophysical chemistry, Max Planck Society, ou_578620              

Content

show
hide
Free keywords: -
 Abstract: Evidence is presented that glutamate binding to rat brain synaptic plasma membranes measured in Cl(-)- and Na+-free but Ca2+-supplemented buffers is partly due to uptake of glutamate into resealed membrane vesicles. An intravesicular volume of 7.9 microliter/mg protein was measured. Ca2+-induced glutamate binding to synaptic plasma membranes was found to be sensitive to low temperatures as well as to an increase in osmolarity and was abolished by short pulses of ultrasonication. None of several glutamate receptor agonists tested discriminated between basal and Ca2+-induced binding, but 4 out of 5 glutamate uptake inhibitors did. The Ca2+-induced increase in glutamate binding was the same irrespective of whether calcium acetate, calcium sulfate or calcium gluconate in either Tris-acetate of Tris-citrate buffer was used.

Details

show
hide
Language(s): eng - English
 Dates: 1988-02-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/0014-5793(88)80588-X
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: FEBS Letters
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 228 (1) Sequence Number: - Start / End Page: 74 - 78 Identifier: -