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  A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies

Ruprecht, C., Bartetzko, M., Senf, D., Dallabernardina, P., Boos, I., Andersen, M. C. F., et al. (2017). A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies. Plant Physiology, 175(3), 1094-1104. doi:10.1104/pp.17.00737.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-FD1F-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-75F4-7
Genre: Journal Article

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 Creators:
Ruprecht, Colin1, Author              
Bartetzko, Max1, Author              
Senf, Deborah1, Author              
Dallabernardina, Pietro1, Author              
Boos, Irene, Author
Andersen, Mathias C. F., Author
Kotake, Toshihisa, Author
Knox, J. Paul, Author
Hahn, Michael G., Author
Clausen, Mads H, Author
Pfrengle, Fabian1, Author              
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1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

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 Abstract: In the last three decades, more than 200 monoclonal antibodies have been raised against most classes of plant cell wall polysaccharides by different laboratories world-wide. These antibodies are widely used to identify differences in plant cell wall components in mutants, organ and tissue types, and developmental stages. Despite their importance and broad use, the precise binding epitope for only a few of these antibodies has been determined. Here, we use a plant glycan microarray equipped with 88 synthetic oligosaccharides to comprehensively map the epitopes of plant cell wall glycan-directed antibodies. Our results reveal the binding epitopes for 78 arabinogalactan-, rhamnogalacturonan-, xylan-, and xyloglucan-directed antibodies. We demonstrate that, with knowledge of the exact epitopes recognized by individual antibodies, specific glycosyl hydrolases can be implemented into immunological cell wall analyses, providing a framework to obtain structural information on plant cell wall glycans with unprecedented molecular precision.

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 Dates: 2017-092017-11
 Publication Status: Published in print
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 Identifiers: DOI: 10.1104/pp.17.00737
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Grant ID : PF850/1-1
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Funding organization : DFG

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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 175 (3) Sequence Number: - Start / End Page: 1094 - 1104 Identifier: ISSN: 0032-0889