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  Cryo-electron tomography-the cell biology that came in from the cold

Wagner, J., Schaffer, M., & Fernandez-Busnadiego, R. (2017). Cryo-electron tomography-the cell biology that came in from the cold. FEBS Letters, 591(17), 2520-2533. doi:10.1002/1873-3468.12757.

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 Creators:
Wagner, Jonathan1, Author              
Schaffer, Miroslava1, Author              
Fernandez-Busnadiego, Ruben1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: TRANSMISSION ELECTRON-MICROSCOPY; TRIPEPTIDYL-PEPTIDASE-II; CRYO-LIGHT MICROSCOPY; BEAM-INDUCED MOTION; MEAN FREE-PATH; IN-SITU; MOLECULAR ARCHITECTURE; STRUCTURAL-ANALYSIS; PHASE-CONTRAST; MULTICELLULAR ORGANISMSBiochemistry & Molecular Biology; Biophysics; Cell Biology; Cryo-EM; cryo-FIB; protein aggregation;
 Abstract: Cryo-electron tomography (cryo-ET) provides high-resolution 3D views into cells pristinely preserved by vitrification. Recent technical advances such as direct electron detectors, the Volta phase plate and cryo-focused ion beam milling have dramatically pushed image quality and expanded the range of cryo-ET applications. Cryo-ET not only allows mapping the positions and interactions of macromolecules within their intact cellular context, but can also reveal their in situ structure at increasing resolution. Here, we review how recent work using cutting-edge cryo-ET technologies is starting to provide fresh views into different aspects of cellular biology at an unprecedented level of detail. We anticipate that these developments will soon make cryo-ET a fundamental technique in cell biology.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000409313500003
DOI: 10.1002/1873-3468.12757
 Degree: -

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Project name : FP7 GA ERC-2012-SyG_318987–ToPAG
Grant ID : 318987
Funding program : Funding Programme 7 (FP7)
Funding organization : European Commission (EC)

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: SI: Jerusalem Special Issue Volume / Issue: 591 (17) Sequence Number: - Start / End Page: 2520 - 2533 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501