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  SRpHi ratiometric pH biosensors for super-resolution microscopy.

Richardson, D. S., Gregor, C., Winter, F., Urban, N. T., Sahl, S. J., Willig, K. I., et al. (2017). SRpHi ratiometric pH biosensors for super-resolution microscopy. Nature Communications, 8: 577. doi:10.1038/s41467-017-00606-4.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-EEF9-F Version Permalink: http://hdl.handle.net/21.11116/0000-0001-49D0-2
Genre: Journal Article

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 Creators:
Richardson, D. S.1, Author              
Gregor, C.1, Author              
Winter, F.1, Author              
Urban, N. T.1, Author              
Sahl, S. J.1, Author              
Willig, K. I.1, Author              
Hell, S. W.1, Author              
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1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.

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Language(s): eng - English
 Dates: 2017-09-18
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-017-00606-4
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Title: Nature Communications
Source Genre: Journal
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Pages: 9 Volume / Issue: 8 Sequence Number: 577 Start / End Page: - Identifier: -